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Laryngeal polyp associated with reflux disease: a case report
Journal of Medical Case Reports volume 14, Article number: 2 (2020)
Among the most common benign laryngeal lesions are vocal nodules and polyps. Their etiology is related to vocal abuse. Gastroesophageal reflux disease is a common condition presenting with a broad spectrum of symptoms, among which are extraesophageal manifestations such as laryngeal polyps.
A 24-year-old Middle Eastern woman presented to the author’s institution with dysphonia and dyspepsia. She underwent endoscopy and was diagnosed with severe reflux disease. In addition, laryngoscopy revealed a polyp at the left vocal cord, and the patient underwent polypectomy. Histopathological examination revealed a laryngeal polyp of telangiectatic type characterized by hyperplastic epithelial covering with reactive atypia, prominent superficial acanthosis with neutrophils, and prominent chronic inflammation and thrombosed vessels in the stroma.
This report focuses on the pathological findings associated with a laryngeal polyp in a young patient diagnosed with severe reflux disease. Acknowledging such characteristic changes in a laryngeal polyp could aid in the diagnosis of gastroesophageal reflux disease.
Laryngeal polyp is defined pathologically as a noninflammatory response to laryngeal injury usually caused by vocal cord abuse and irritation . “Vocal abuse” refers to misuse of vocal behaviors leading eventually to trauma of the laryngeal mucosa, such as by excessive talking, prolonged and excessive loudness, and the use of inappropriate pitch . Thus, it is more common in singers . The most common clinical manifestation is voice change: generalized and persistent hoarseness, change in voice quality, and increased effort in producing the voice . The usual location of polyps on the superior surface of the cord makes it easy to visualize them by video stroboscopic examination . There are several other causes of laryngeal polyps, despite being less common, such as gastroesophageal reflux disease (GERD), and chronic inhalation of irritants (such as industrial fumes and cigarette smoke). GERD symptoms are described as typical if they present with digestive symptoms (e.g., pyrosis, regurgitation, back breastbone pain) and atypical if the symptoms are extraesophageal, related to the larynx, the pharynx, or other respiratory airways (e.g., cough, dysphonia, dysphagia) [4, 5]. The causes of GERD are multifactorial and include many well-described factors , all of which are associated with transient lower esophageal sphincter relaxation that allows a bolus of refluxate to move from the stomach into the esophagus . One of the defense mechanisms in combating gastric reflux is the upper esophageal sphincter (cricopharyngeus), whose pressure might fail to increase in response to esophageal acid exposure, leading to laryngopharyngeal reflux (LPR) . The exact etiology behind the failure of the upper sphincter is uncertain; however, it is also clear that such failure is reversible with effective antireflux treatment . LPR has been associated with vocal cord polyps, vocal cord granulomas, laryngospasm, laryngeal carcinoma, and subglottic stenosis . We report a case of vocal cord polyp diagnosed in a patient with severe GERD.
A 24-year-old Middle Eastern woman presented to the author’s ear, nose, and throat (ENT) clinic with a complaint of dysphonia that had been present for 2 months. The patient gave no history of any intubations, trauma, or voice abuse. She had associated dyspeptic symptoms. On examination of the ear, nose, and throat, a polypoid lesion was present on the middle one-third region of the left vocal cord measuring approximately 4 × 3 cm in diameter. The patient was referred to the gastroenterology clinic to undergo endoscopy. She was diagnosed with grade C GERD on the basis of the Los Angeles classification system of GERD. Returning to the ENT clinic, the patient was scheduled for surgery for removal of the laryngeal polyp. With the patient under general anesthesia, the polypoid lesion was excised completely by suspension direct laryngoscopy, with no residual tissue left behind.
The excised polyp was fixed in 10% formalin and embedded in paraffin. Serial sections, 5 μm thick, were processed for hematoxylin and eosin (H&E) staining. Immunohistochemistry (IHC) staining was carried out with the streptavidin-biotin method. Primary antibodies (Additional file 1: Table S1) were purchased from Genemed Biotechnologies (San Francisco, CA, USA).
On gross pathological examination, the specimen consisted of a polypoid mass measuring 4 × 3 cm with a smooth outer surface and a congested cut section. Histologically, as observed by H&E) staining, the polyp was lined with thickened, nonkeratinized, stratified, squamous epithelial covering with disruption of superficial layers of epithelium causing small blisters with intraepidermal microabscesses and underlying areas of hemorrhage (Fig. 1a). The basal epithelial cells were thickened with elongation of rete ridges and mild atypical changes involving the lower third of the epithelial covering in the form of enlarged hyperchromatic nuclei and mild disturbed polarity (Fig. 1b). The underlying stroma showed numerous proliferated blood vessels (Fig. 1c), with some of them dilated with congestion and others with thrombi. Nests of uniform stratified squamous epithelium were present within the stroma and surrounded with basement membrane, representing pseudoepitheliomatous hyperplasia. Dense fibrous tissue deposition was present in the stroma, with focal areas of edema and chronic inflammatory cell infiltrate and numerous focal accumulations of clear cells with enlarged nuclei. Focal separation of overlying epithelial covering by edema was seen (Fig. 1d). Focal ulceration with underlying granulation tissue-like reaction was also observed.
By IHC staining, a negative reaction to p53 in the overlying epithelium was detected, thus excluding the possibility of epithelial dysplasia. In addition, E-cadherin expression was decreased in the superficial layers of the squamous epithelial covering, leading to intercellular spongiosis and the accumulation of neutrophils with microabscess formation. The accumulated clear cells stained positive for CD68 and negative for pan-cytokeratin, confirming their nature as foamy macrophages.
Discussion and conclusions
Vocal cord polyps and nodules are among the most common prevalent laryngeal lesions (1.0–1.7%), with a slight female and pediatric predominance . They are typically caused by vocal overuse, whereas other irritants may contribute to the development of polyps, such as GERD, smoking, and aspiration of chemical substances . Differentiation between vocal cord nodules and polyps can be done on the basis of both laryngoscopic and pathological parameters . From a histological point of view, vocal cord polyps are classified as telangiectatic and gelatinous polyps. Table 1 summarizes the laryngoscopic and pathological features of vocal cord nodules and polyps [10, 11]. On the basis of these data, the present case represents a vocal cord telangiectatic polyp. GERD has been implicated in the pathogenesis of vocal cord nodules and polyps, possibly due to adductory collision of the vocal cord by the effect of reflux, which results in local trauma, inflammation, and irritation . Moreover, higher presence of pepsin was reported in patients with vocal cord polyps than in a control group . Other laryngeal complications of GERD include paradoxical vocal fold motion, laryngospasm, laryngeal granuloma, stenosis, strictures, ulcers, chronic laryngitis, and laryngeal carcinoma [6, 13,14,15,16]. It has been estimated that 55–79% of patients who present with unresponsive hoarseness resulting from chronic laryngitis have acid reflux .
The present patient had the clinical and pathological features of a vocal cord polyp associated with GERD. The clinical features included dysphonia associated with dyspepsia. The pathological features included epithelial changes; thickening of the epithelial lining with microabscesses, reactive atypia, pseudoepitheliomatous hyperplasia, and stromal changes; vascular proliferation; hemorrhage; thrombosis edema; fibrous deposition and increased number of foamy macrophages. Despite the presence of atypical epithelial cell changes, a negative reaction to p53 excluded the possibility of dysplasia [17, 18]. On the one hand, acknowledging such a spectrum of changes in a laryngeal polyp could aid in the diagnosis of associated GERD. On the other hand, smoking-associated vocal cord polyps are characterized by significant epithelial hyperplasia, leukoplakia, thickened basement membrane, hyaline degeneration, and lamina propria edema [9, 11].
The preferred treatment in all patients with larynx-associated GERD features is aggressive antireflux therapy combined with speech therapy . Marked improvement could be achieved in the associated laryngeal lesions, such as granulomas and subglottic stenosis, with antireflux therapy alone . Patients with laryngeal polyps, as in the present patient, require surgical intervention for removal of the polyp to avoid airway obstruction and should be given aggressive antireflux therapy preoperatively and maintained on it postoperatively .
Regarding the present patient, following laryngeal polypectomy, she was treated with both a proton pump inhibitor (1 mg/kg body mass) and histamine H2 receptor antagonists (1 mg/kg body mass) for 6 months in addition to erythromycin (500 mg twice daily) for 5 days, and an antireflux diet was prescribed by the gastroenterologist. After treatment, the patient was assessed, and she showed dramatic improvement. The patient remains under constant follow-up in a gastrological and laryngological outpatient clinic.
Availability of data and materials
Data sharing is not applicable to this article, as no datasets were generated or analyzed during the current study.
Wallis L, Jackson-Menaldi C, Holland W, Giraldo A. Vocal fold nodule vs. vocal fold polyp: answer from surgical pathologist and voice pathologist point of view. J Voice. 2004;18(1):125–9.
Cipriani NA, Martin DE, Corey JP, et al. The clinicopathologic spectrum of benign mass lesions of the vocal fold due to vocal abuse. Int J Surg Pathol. 2011;19(5):583–7.
Cho KJ, Nam IC, Hwang YS, et al. Analysis of factors influencing voice quality and therapeutic approaches in vocal polyp patients. Eur Arch Otorhinolaryngol. 2011;268(9):1321–7.
Hom C, Vaezi MF. Extra-esophageal manifestations of gastroesophageal reflux disease: diagnosis and treatment. Drugs. 2013;73(12):1281–95.
Mosca F, Rossillo V, Leone CA. Manifestations of gastro-pharyngo-laryngeal reflux disease. Acta Otorhinolaryngol Ital. 2006;26(5):247–51.
Koufman JA. The otolaryngologic manifestations of gastroesophageal reflux disease (GERD): a clinical investigation of 225 patients using ambulatory 24-hour pH monitoring and an experimental investigation of the role of acid and pepsin in the development of laryngeal injury. Laryngoscope. 1991;101(4 pt 2 Suppl 53):1–78.
Koufman JA. Perspective on laryngopharyngeal reflux: from silence to omnipresence. classics in voice and laryngology. In: Branski R, Sulica L, editors. Classics in voice and laryngology. San Diego: Plural Publishing; 2009. p. 179–266.
Koufman JA. Low-acid diet for recalcitrant laryngopharyngeal reflux: therapeutic benefits and their implications. Ann Otol Rhinol Laryngol. 2011;120(5):281–7.
Vasconcelos D, Gomes A, Araújo C. Vocal fold polyps: literature review. Int Arch Otorhinolaryngol. 2019;23(1):116–24.
Bohlender J. Diagnostic and therapeutic pitfalls in benign vocal fold diseases. GMS Curr Top Otorhinolaryngol Head Neck Surg. 2013;12:Doc01. https://doi.org/10.3205/cto000093.
Nunes RB, Behlau M, Nunes MB, Paulino JG. Clinical diagnosis and histological analysis of vocal nodules and polyps. Braz J Otorhinolaryngol. 2013;79(4):434–40. https://doi.org/10.5935/1808-8694.2013007.
Kuhn J, Toohill RJ, Ulualp SO, et al. Pharyngeal acid reflux events in patients with vocal cord nodules. Laryngoscope. 1998;108(8 Pt 1):1146–9.
Postma GN, Halum SL. Laryngeal and pharyngeal complications of gastroesophageal reflux disease. GI Motil Online. 2006; https://doi.org/10.1038/gimo46.
Vaezi MF, Hicks DM, Abelson TI, Richter JE. Laryngeal signs and symptoms and gastroesophageal reflux disease (GERD): a critical assessment of cause and effect association. Clin Gastroenterol Hepatol. 2003;1(5):333–44.
Koufman JA, Amin MR, Panetti M. Prevalence of reflux in 113 consecutive patients with laryngeal and voice disorders. Otolaryngol Head Neck Surg. 2000;123(4):385–8.
Little FB, Koufman JA, Kohut RI, Marshall RB. Effect of gastric acid on the pathogenesis of subglottic stenosis. Ann Otol Rhinol Laryngol. 1985;94:516–9.
Gusterson BA, Anbazhagan R, Warren W, et al. Expression of p53 in premalignant and malignant squamous epithelium. Oncogene. 1991;6(10):1785–9.
Mondal D, Saha K, Datta C, et al. Ki67, p27 and p53 expression in squamous epithelial lesions of larynx. Indian J Otolaryngol Head Neck Surg. 2013;65(2):126–33.
The author extends special thanks to M. Saad for his technical skills in preparing histopathological slides.
All pathological studies in this report were funded by the author.
Ethics approval and consent to participate
This study was approved by the committee of ethics in the Faculty of Medicine, Suez Canal University, Egypt (no. 12457).
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Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
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Hassan, W.A. Laryngeal polyp associated with reflux disease: a case report. J Med Case Reports 14, 2 (2020) doi:10.1186/s13256-019-2324-0
- Laryngeal polyp
- Gastroesophageal reflux disease
- Pathological changes