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Mycobacterium tuberculosis as a cause of mandibular osteomyelitis in a young woman: a case report

  • Jorge Tellez-Rodriguez1,
  • Rubi Lopez-Fernandez1,
  • Rodolfo Rodriguez-Jurado2,
  • Hayde Nallely Moreno-Sandoval3,
  • Francisco Martinez-Perez4 and
  • Juan Antonio Gonzalez-Barrios3Email author
Journal of Medical Case Reports201610:366

https://doi.org/10.1186/s13256-016-1118-x

Received: 6 May 2016

Accepted: 24 October 2016

Published: 20 December 2016

Abstract

Background

Tuberculosis is considered an emerging disease worldwide; in the last 10 years, its incidence has increased to more than 9.6 million cases of active tuberculosis. In 2014, it resulted in 1.5 million patient deaths. However, oral presentation with bone involvement occurs in less than 3% of all reported cases and rarely arouses clinical suspicion on initial presentation.

Case presentation

A 15-year-old Mexican girl who had a previous diagnosis of neurofibromatosis presented to our hospital with pain and swelling in the region of the left mandibular body since November 2011. A clinical examination revealed pain in the mandibular region, a mass of soft consistency that seemed to involve bone, and a fistula with discharge of intraoral purulent material. Additionally, tachycardia and hyperthermia were observed. The left submental and submandibular regions had a 12-cm-diameter swelling, which was well-delineated and nonerythematous. The final diagnosis was established by real-time polymerase chain reaction.

Conclusions

The final diagnosis of rare cases of tuberculous osteomyelitis in the jaw can be established by deoxyribonucleic acid (DNA) identification of Mycobacterium tuberculosis in the lesion. Simple and fast complementary diagnosis by real-time polymerase chain reaction is a fundamental approach to establishing early and effective pharmacological and surgical treatment.

Keywords

Tuberculosis Osteomyelitis Mycobacterium tuberculosis Jaw Case report

Background

Tuberculosis is considered an emerging disease worldwide. The World Health Organization estimated that the incidence of tuberculosis increased in the last 10 years to more than 9.6 million cases of active tuberculosis, and that it caused 1.5 million patient deaths in 2014. Approximately 1 million children were infected by Mycobacterium tuberculosis and 140,000 children died as a result of tuberculosis. By the end of 2014, an additional 480,000 cases of multidrug-resistant tuberculosis were reported worldwide [1]. Tuberculosis is rarely confined to bone and develops frequently secondary to a systemic infection. However, oral presentation with bone involvement occurs in less than 3% of all reported cases; thus, it rarely arouses clinical suspicion at initial presentation. Mandibular osteomyelitis caused by M. tuberculosis infection is a rare medical condition. Because tuberculosis infection is rarely confined to bone tissue, such a manifestation may be considered as an extrapulmonary complication.

Adults are more frequently affected, although cases in children have also been described [25]. Tuberculous lesions of the oral cavity are quite rare; despite the high incidence of the systemic disease, they can be explained by M. tuberculosis inhibition by the salivary components [6]. The mechanism of propagation of tuberculosis infection to the mandibular bone can be by direct inoculation through dental extraction, lesions of the mucosa during teeth eruption, and spread from adjacent tissue or via a hematogenous route [7]. The clinical presentation and radiological imaging of mandibular tuberculosis are similar to those of chronic secondary osteomyelitis and regular dentoalveolar abscess. However, cervical lymphadenopathy produces discrete or diffuse masses, which are often not sensitive to palpation. These can be distinctive features in some patients [8]. This similarity to conventional cases of osteomyelitis emphasizes the importance of considering a differential diagnosis of tuberculous osteomyelitis of the jaw, especially if the patient has a suspicious medical record for tuberculosis [9, 10]. The clinical lesions of oral tuberculosis present as painful and irregular ulcers, especially in the posterior tongue area, pharynx, or palate, and they are frequently secondary to active pulmonary tuberculosis [11]. Bone involvement of the maxilla and mandible can produce tuberculous osteomyelitis by occult spread of the microorganism [12], and regional lymphadenopathy usually accompanies the oral lesions. For this reason, inclusion of scrofula in the differential diagnosis is obligatory [13]. Sometimes, bone invasion can lead to tuberculosis-related osteomyelitis. The incidence is higher in poor and densely populated areas, areas of low socioeconomic status, and in human immunodeficiency virus (HIV)-infected and immunocompromised individuals [11]. The differential diagnosis of oral tuberculosis includes other important diseases, such as squamous cell carcinoma; primary syphilis and various oral lesions, such as actinomycosis; fungal lung diseases, such as histoplasmosis, coccidioidomycosis, and blastomycosis; lymphoma; and submandibular sialadenitis [11]. It is important to remember that trauma is the leading cause of oral ulcers and should be included in the differential diagnosis, and all oral ulcers that raise suspicion of tuberculosis require biopsy to exclude cancer.

Radiographic features of acute osteomyelitis are usually not immediately apparent. First, the exudate progresses through the soft tissue component through the preexisting medullary spaces. Until the trabecular bone components have resorbed significantly, the magnitude of the destruction will be evident on radiographs as macular, mottled, or fuzzy in appearance with feathered edges and accompanied by unresorbed fragments of dead bone surrounded by large purulent areas. The chronic osteomyelitis area has a mottled appearance and is radio-opaque, which is designated as osteosclerosis that can be limited to the surrounding tooth root area. In other cases, it can induce osteosclerosis of larger areas or areas of edentulous bone [14]. M. tuberculosis induces a specific response in infected tissues that are characterized by local areas of macrophages surrounded by lymphocytes and fibroblasts, where the macrophages develop an abundant eosinophilic cytoplasm similar to the epithelial cells that are known to be like epithelioid cells. The fusion of macrophages gives them the appearance of giant Langerhans cells in which the nuclei are distributed in episomal form, characteristic of the histological diagnosis of bone tuberculosis [12].

Case presentation

A 15-year-old Mexican girl was referred to our hospital with a previous diagnosis of neurofibromatosis (NF), and reported pain and swelling in the region of the left mandibular body for the last 5 months. Her clinical history revealed antecedents of NF type 1, and her clinical examination disclosed pain in the mandibular region during palpation, a mass of soft consistency that seemed to involve bone, with a fistula and discharge of intraoral purulent material, tachycardia, and hyperthermia (39 °C). The left submental and submandibular regions had a 12-cm-diameter swelling and well-delineated, nonerythematous hyperthermia. An intraoral examination showed tooth mobility and few purulent exudate outputs. Laboratory studies provided the following relevant results: hemoglobin 12 g/dl, hematocrit 36.7%, and leukocytes 6.9 × 103/μl. The differential white blood cell count showed neutrophils 4.2 × 103/μl, lymphocytes 1.3 × 103/μl, monocytes 1.1 × 103/μl, eosinophils 0.3 × 103/μl, basophils 0.09 × 103/μl, and platelets 192 × 103/μl. Computed tomography (CT) (Fig. 1a and b) and three-dimensional reconstruction by CT showed erosion and perforations of cortical periosteal bone formation of the left hemimandible (Fig. 1c and d). Single-photon emission computed tomography (SPECT) coupled with CT performed with technetium-99 m-ciprofloxacin (SPECT/CT 99mTc-ciprofloxacin) revealed an abnormally concentrated area of radiolabeled antibiotic at the left mandible, right elbow, and soft tissues of the right hand, suggesting a disseminated infectious process (Fig. 2a–c). An intraoral secretion bacteriological culture isolated Streptococcus parasanguinis and high-level gentamicin- and vancomycin-resistant Enterococcus faecium. An antibiogram shows resistance to ampicillin, erythromycin, vancomycin, cefazolin, gentamicin, streptomycin, levofloxacin, oxacillin, and penicillin G, as well as sensitivity to daptomycin, doxycycline, linezolid, moxifloxacin, nitrofurantoin, quinupristin/dalfopristin, and rifampicin (RIF).
Fig. 1

Clinical imaging of our patient with tuberculous osteomyelitis in the left mandible showing left-sided cheek swelling. (a) Frontal and (b) lateral views displaying mandible tuberculosis symptoms. (c) Frontal and (d) lateral views of three-dimensional reconstruction by computed tomography showing erosion and perforations of the cortical periosteal bone

Fig. 2

Technetium-99 m-ciprofloxacin accumulation in the mandible. Images show an abnormal concentrated area of radiolabeled antibiotic at the level of the left mandible, suggesting an infectious disease process. (a) Frontal view. (b) Oblique view. (c) Lateral view. The arrows indicate the site of 99mTc-Ciprofloxacine accumulation

The presumptive diagnosis was mandibular osteomyelitis. To corroborate the diagnosis, an incisional biopsy was done. The patient had received previous antibiotic administration with procaine benzylpenicillin (600,000 U intravenous therapy) combined with benzylpenicillin sodium (200,000 U intravenous therapy) and metronidazole (500 mg oral therapy) every 8 h for 7 days. Macroscopic and histopathological study revealed an extensive chronic osteomyelitic process (Fig. 3a and b) due to infection that did not cease with antibiotic administration. This raised the possibility of tuberculous osteomyelitis. To identify mycobacteria as a causative agent, molecular diagnostics for tuberculosis was performed for the high diagnostic value [15]. M. tuberculosis molecular identification and drug resistance to isoniazid (INH), RIF, fluoroquinolone (FQ), and aminoglycoside (AG) were done using Anyplex plus mycobacterium tuberculosis/nontuberculous mycobacteria/drug-resistant tuberculosis (MTB/NTM/DR-TB) real-time test (Seegene, Seoul, Korea) in accordance with the manufacturer’s specifications. The polymerase chain reaction (PCR) data confirmed the final molecular diagnosis of disseminated and chronic tuberculous osteomyelitis of the mandible caused by M. tuberculosis (Fig. 3c), and no isoniazid (INH) or rifampicin (RIF) resistance was found by the GenoType multidrug-resistant tuberculosis (MTBDR) test (Hain Lifescience, Nehren, Germany).
Fig. 3

Macroscopic and histological characteristics of jaw and real-time polymerase chain reaction-based diagnosis. a Macroscopic characteristics of inner side of jaw. Large eroded areas accompanied by bleeding and bone decalcification can be seen. b Histopathological characteristics. Extensive chronic osteomyelitis process can be seen. c Molecular diagnosis of tuberculous osteomyelitis of the jaw. Both markers, susceptible Mycobacterium tuberculosis and M. tuberculosis-positive control, conformed to the M. tuberculosis diagnosis. S-MTB Susceptible Mycobacterium tuberculosis biopsy patient sample positive for Mycobacterium tuberculosis, MTB-(C+) Mycobacterium tuberculosis-positive control, NTM-(C+) Nontuberculous mycobacteria-positive control, IC Internal control, NC Negative control

The patient received initial antituberculosis therapy with 300 mg INH, 600 mg RIF, 1.6 g pyrazinamide, and 1 g ethambutol (EMB) for 2 months, followed by maintenance treatment with 300 mg INH and 600 mg RIF. At the sixth month of tuberculosis therapy, the patient developed thrombosis of the inferior alveolar artery that produced extensive jawbone necrosis, which was considered irrecoverable. Thus, an elective hemimandibulectomy was performed, and the surgical defect was reconstructed by titanium plating with the condyle, and 1.2 g ETB was added to the antituberculosis maintenance therapy until 15 months of antifimic drug therapy was complete.

Discussion

NF and tuberculosis rarely coincide in a patient; however, NF type 1 may be accompanied by a common variable immunodeficiency that favors infection by M. tuberculosis and its systemic dissemination [16]. In this paper, we report a case of a young woman with NF and disseminated tuberculosis affecting mainly the jaw that induced inferior alveolar artery thrombosis and irrecoverable mandibular necrosis requiring an elective hemimandibulectomy and completion of antifimic drug therapy.

Although bone tuberculosis, and especially the mandibular presentation [25], remains a rare disease it has great significance because of the significant facial deformity sequelae that occur. However, one of the dilemmas faced by the doctor is the definitive etiologic diagnosis (Table 1). In our patient’s case, different techniques used to support the clinical diagnosis included bacterial cultures, axial CT, and SPECT/CT 99mTc-ciprofloxacin. All results obtained using these diagnostic techniques were indicative of infectious osteomyelitis. The incisional biopsy results were not conclusive for tuberculosis, because they showed only chronic osteomyelitis with an exacerbation process without evidence of granulomatosis. It is well known that all these techniques only have the power to suggest the type of pathology; however, the techniques required to make the diagnosis, one of which is mycobacteria cultivation, are not available in most hospitals. It is well known that this technique requires a long time to reveal the etiology of the disease. Furthermore, an average of 15 days to 5 weeks has typically been required to ultimately make the diagnosis on that basis. In our patient, the Löwenstein-Jensen culture medium was negative at 40 days of cultivation. However, with advances in technology using deoxyribonucleic acid (DNA) and real-time PCR techniques, microorganism identification now occurs in less time (8 h) after sampling. The resolving power that this technique offers is until a bacterial DNA molecule is present in the sample [17]. However, the sample type where M. tuberculosis was identified required a special procedure to amplify the bacterial DNA [15], because the paraffin-embedding process alters the DNA, and the calcium from the bone is capable of inhibiting the PCR enzymes, especially the DNA polymerase. For this reason, it was decided that the quantitative PCR (qPCR) technique should be used to increase the molecular diagnostic sensitivity [18]. For this purpose, a 90-bp segment was amplified, and its amplification was followed by hydrolysis with a TaqMan probe (Applied Biosystems, Foster City, CA, USA). According to the pathology results, the first site of infection manifestation was the mandible. This allowed us to hypothesize that the primary focus was mandibular tuberculosis, subsequently spread via the hematogenous mechanism to the left elbow and the soft tissues of the left hand; however, both foci were developed after mandibular infection without evidence of pulmonary tuberculosis. All tuberculosis infection foci also were corroborated by the qPCR technique.
Table 1

Comparative characteristics of reported tuberculous osteomyelitis of the jaw during infancy

Sex, age [reference]

Clinical findings

Mandibular Region

Histological diagnosis

Methods for tuberculosis diagnosis

Treatment (follow-up)

Male, 3 years old [2]

1 month

Left submandibular mass

Lymph enlargement

Angle

Granulomatous osteomyelitis consistent with tuberculosis

Primary.

Ziehl-Neelsen staining and culturing not performed

Antitubercular therapy (6 months)

Without recurrence

Male, 4 years old [3]

1 month

Progressive swelling on cheek, right side

Caries in the right second primary molar

Lymph nodes

Body

Angle

Granulomatous osteomyelitis

Secondary

Granulomatous area

Giant and epithelioid cells surrounded by lymphocytes and plasma cells

Positive Ziehl-Neelsen staining

Antitubercular chemotherapy (6 months)

Complete resolution

Female, 10 years old [4]

2 months

Gradual swelling in left mandibular region

Caries in the lower left second deciduous molar

No evidence of lymphadenopathy

Body

Caseous granuloma

Primary

Fine-needle aspiration cytology of swelling on left side of mandible

Antitubercular chemotherapy for 8 months

Without recurrence

Female, 14 years old [5]

2 months

Progressive swelling in the right parotid region

Tooth extraction 8 months ago

Angle

Epithelioid cell granuloma with caseous necrosis

Giant cell, Langerhans type

Peripheral mantle of lymphocytes

Primary

Open biopsy

Antitubercular therapy

Complete resolution

Female, 18 years old [6]

8 months

Edema and trismus (preauricular region) of left side of face

Ulcerative lesion (retromolar region)

Lymph node enlargement

Ramus

Condyle

Caseous granuloma

Primary

Fine-needle aspiration cytology

Not started

Female, 9 years old [7]

3 months

Progressive mandibular edema Intermittent fever

Lymph node

Angle

Ramus

Multiple epithelioid cell granulomas

Multinucleated giant cells

Caseation necrosis areas

Lymphocytic infiltrates

Primary

Positive Ziehl-Neelsen staining for AFB

Antitubercular treatment (9 months)

No recurrence

Female, 16 years old [8]

6 months

No important antecedents

No tooth extractions or any oral trauma

Ramus

Condyle

Granulation tissue

Necrotic bone

Focal epithelioid cell granulomas

Langerhans giant cells

Chronic inflammatory cells

Primary

Negative sputum for Ziehl-Neelsen staining

Positive tuberculin skin test

Antitubercular treatment (9 months)

No recurrence

Female, 16 years old

[present report]

7 months

Left mandibular inflammation

Ulcerative lesion over the retromolar region (impacted 37)

Lymph node

Body

Ramus

Condyle

Chronic osteomyelitic process

Primary

Molecular diagnosis (real-time PCR)

Antitubercular treatment (9 months)

Hemimandibulectomy

Mandibular reconstruction

No recurrence (15 months)

AFB Acid-fast bacilli, PCR Polymerase chain reaction

Our patient’s case highlights the clinical difficulty in establishing the etiology and simultaneously proposes the use of molecular diagnostics on the basis of clinical suspicion [25]. However, we are aware that not all hospitals have access to these advanced molecular diagnostic techniques. Establishment of an etiological diagnosis is the main goal of the clinic, with an aim to set up definitive treatment. Because a positive test for M. tuberculosis is necessary to initiate drug therapy in Mexico, as treatment is provided by the government free of charge for the patient, our patient underwent left hemimandibulectomy and placement of a mandibular reconstruction plate in the left jaw, accompanied by a three-drug antifimic scheme including INH (300 mg/d), RIF (600 mg/d), and ETB (1.2 g/d) 9 months after surgery, to which the patient responded satisfactorily. The hemimandibulectomy was considered as an optimal treatment because the patient had developed thrombosis of the inferior alveolar artery that had produced extensive jawbone necrosis, which was considered irrecoverable with the antituberculosis therapy. Currently, the patient is healthy and recovering, without indication of systemic tuberculosis. The clinical, surgical, and pharmacological results have allowed the patient to return to her daily activities, normal social life, and academic activities.

Conclusions

The combination of NF and tuberculosis of the jaw is an extremely rare clinical condition with a high diagnostic difficulty grade. In our patient, the molecular diagnosis by qPCR played a key role in establishing the final diagnosis and surgical treatment as indicated, which allowed the patient to resume her normal activities and social relationships. Although primary tuberculous osteomyelitis of the jaw is a rare condition, it should be considered in the differential diagnosis of mandibular lesions in comparable clinical situations.

Patient’s perspective

My parents and I are very happy and grateful for the care that I received from the medical staff of Pediatrics National Institute, Genomic Medicine Laboratory of the “October 1st” Regional Hospital, and in general, to all professionals who were involved to finally get my diagnosis, and mainly for solving my health problem.

Abbreviations

99mTc: 

Technetium-99 m

CT: 

Computed tomography

MTB: 

Mycobacterium tuberculosis

NTM: 

Nontuberculous mycobacteria

PCR: 

Polymerase chain reaction

qPCR: 

Quantitative polymerase chain reaction

SPECT: 

Single-photon emission computed tomography

TB: 

Tuberculosis

EMB: 

Ethambutol

INH: 

Isoniazid

RIF: 

Rifampicin

FQ: 

Fluoroquinolone

AG: 

Aminoglycoside

S-MTB: 

Susceptible Mycobacterium tuberculosis

MTB-(C+): 

Mycobacterium tuberculosis-positive control

NTM-(C+): 

Nontuberculous mycobacteria-positive control

IC: 

Internal control

NC: 

Negative control

NF: 

Neurofibromatosis

AFB: 

Acid-fast bacilli

Declarations

Acknowledgements

We thank Dr. Paul Miranda-Aguilar and Dr. Eva Portillo Nava for their participation in the patient’s treatment and their invaluable comments on the manuscript. We especially thank the patient’s parents for allowing us to publish this case report.

Funding

The project was funded by Solidarity and Social Investment Fund/Consejo Nacional de Ciencia y Tecnología (FOSIS-CONACYT) México grant S0008-2012-1 and Fondo Sectorial de Investigación en Salud y Seguridad Social (FONSEC SSA/IMSS/ISSSTE) grant 000000000181384.

Availability of data and materials

All materials described in this article are readily available to any scientist wishing to use them.

Authors’ contributions

RLF and JTR treated the patient and prepared the case report. RRJ performed the histopathological study and suggested the tuberculosis diagnosis. HNMS and FMP performed the molecular diagnosis. JAGB was the scientific advisor, conceived of and prepared the final version of this manuscript, and prepared the figures. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Written informed consent was obtained from the patient’s legal guardian(s) for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.

Ethics approval and consent to participate

Not applicable.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Departamento de Cirugía Maxilofacial
(2)
Departamento de Anatomía Patológica del Instituto Nacional de Pediatría
(3)
Laboratorio de Medicina Genómica, Hospital Regional “1o. de Octubre”
(4)
Laboratorio de Genómica de Celomados, Grupo de Investiagición en Microbiología y Genética, Escuela de Biología, Universidad Industrial de Santander

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Copyright

© The Author(s). 2016

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