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Epithelioid cell granuloma with caseating necrosis possibly caused by periapical periodontitis: a case report
© The Author(s). 2018
- Received: 18 July 2018
- Accepted: 24 October 2018
- Published: 11 December 2018
Epithelioid cell granuloma with caseating necrosis is a typical pathological finding in tuberculosis. While specific inflammation, including that related to tuberculosis, can induce caseating granuloma formation, there have been very few reports on the induction of caseating granuloma by non-specific inflammation. Chronic periapical periodontitis is usually related to bacterial biofilm formation as well as fungal or viral infection in the periapical lesion. However, it is difficult to eliminate these extraradicular pathogenic microbes by normal endodontic therapy alone, and more invasive surgical removal is almost always required.
Here we describe the case of a 30-year-old Japanese woman who had suffered from dull pain related to periapical periodontitis for approximately 10 years. Although the causal tooth had been previously extracted at the Department of Oral Surgery of another hospital in 2015, inflammation of the surrounding tissue had not abated. She was referred to our hospital in May 2016 and underwent surgical debridement via an intra/extraoral approach under general anesthesia. A caseating granuloma accompanied by a small amount of fungi was histopathologically confirmed in the excised specimen. Her inflammation has not been exacerbated since the operation.
This is the first report in which non-specific inflammation is shown to induce caseating granuloma arising in the jaw. Our report also highlights the importance of sufficient root canal treatment during the first stage of the procedure.
- Epithelioid cell granuloma
- Caseating necrosis
- Periapical periodontitis
Epithelioid cell granuloma accompanied by Langhans multinucleated giant cells and enclosing caseating necrosis is a typical pathological finding in tuberculosis. In the head and neck area, tuberculosis commonly develops in the oral mucosa, especially the gingiva, and in the lymph nodes. Tubercles are rarely seen in the jaw, as tuberculous osteomyelitis occurs in < 2% of patients with skeletal tuberculosis . However, epithelioid cell granuloma with caseating necrosis may also develop at sites of intense non-specific inflammation, albeit rarely. Furthermore, there has been no report of non-specific inflammation causing an epithelioid cell granuloma with caseating necrosis in the jaw, although involvement of the long bones has been reported.
Among the causes of persistent periapical periodontitis is endodontic failure due to defective instruments, perforations, overfilling, underfilling, ledges, and root canal therapies with iatrogenically altered root canal morphology . In the affected teeth, bacterial biofilm formation as well as fungal and viral infections of the periapical lesion are likely [3–7]. These infections are difficult to eradicate by routine endodontic treatment, and surgical curettage, in some cases including tooth extraction, may be the only possible cure.
Here we describe a case of long-term periapical periodontitis in which overextended gutta-percha possibly induced the formation of an epithelioid cell granuloma with caseating necrosis.
Epithelioid cells are activated macrophages with a shape similar to that of epithelial cells. Epithelioid cell granuloma may occur at sites of intense inflammation and may be caseating or non-caseating. The caseating type occurs in the setting not only of specific inflammation, including tuberculosis and syphilis, but also of non-specific inflammation. The non-caseating type is seen in patients with sarcoidosis and leprosy, among other diseases.
Our patient did not present with the clinical findings typical of tuberculosis, that is, cough, sputum, and lymphadenopathy. She also had no abnormal findings on her chest X-ray and blood test. The pain had persisted for approximately 10 years, and there was no family history of tuberculosis. Acid-fast bacilli were not detected. Together, these findings ruled out tuberculosis. Syphilis was excluded by blood test.
There are very few reports in which a caseating epithelioid cell granuloma was induced by non-specific inflammation. Szilagyi et al.  reported the occurrence of a granuloma with caseation and Langhans-type giant cells in a tibia infected by Salmonella typhi, which was healed by saucerization. Assari et al.  described a chronic granulomatous reaction with caseating necrosis in the ulnar and radius of a patient with chronic recurrent multifocal osteomyelitis. The lesions were negative for bacteria, acid-fast bacilli, and fungi and were healed by non-steroid anti-inflammatory drugs and bisphosphonate.
In our patient with clinically diagnosed osteomyelitis of the mandible we chose treatment via surgical debridement. Osteomyelitis in the head and neck is induced by various factors. The incidence of osteomyelitis in the mandible and maxilla of patients with odontogenic predisposing factors is reported to be 22% and 30%, respectively . Odontogenic osteomyelitis is usually induced by periodontitis. Chronic periapical periodontitis was shown to arise in association with an extraradicular bacterial biofilm located on an overextended gutta-percha surface or the cementum surface of the root against a background of periodontitis . Planktonic bacteria emerging from biofilms may induce acute inflammation, but they are, for the most part, susceptible to antibiotics. However, bacteria encased in biofilms are resistant to antibiotics and to immune system attack and may therefore linger, even over the course of a lifetime . Porphyromonas gingivalis, Tannerella forsythensis, and Prevotella intermedia are often detected in periapical biofilms , but many of the bacteria at these sites are, as yet, unidentified because they are uncultivative. While periapical periodontitis is mostly induced by infection of oral bacteria, fungi and viruses may also be involved. Fungi are isolated from the lesions of approximately 7% of patients with therapy-resistant periapical-periodontitis, with Candida albicans as the most commonly isolated species . The presence of Epstein–Barr virus and human cytomegalovirus in chronic symptomatic periapical lesions has also been reported [6, 7]. Currently, the most effective treatment of bacterial biofilms and fungal and viral infections in a periapical lesion is surgical removal, although new therapies are being developed .
In our patient, periapical periodontitis might have induced the epithelioid cell granuloma with caseating necrosis, given the chronic dull pain that developed even after the root canal treatment of tooth #47. A dental X-ray before the extraction of tooth #47 showed insufficient root canal filling. We previously reported that Garrè’s osteomyelitis induced by periapical infection could be healed by a root canal procedure . Had our patient presented with acute inflammation, she could have undergone retreatment, including curettage of the periapical granulation tissue with or without appendectomy or intentional replantation. Early treatment would have enabled the preservation of teeth #47 and #46 and avoided caseating granuloma formation. Acute inflammation might also have been controlled by our patient’s innate/acquired immune system, as she was young and healthy.
This is the first report of a patient with neglected periapical periodontitis that may have induced an epithelioid cell granuloma with caseating necrosis in intra/extra mandibular bone. Treatment consisted of surgical enucleation but also required extraction of the vital adjacent tooth. In this patient, endodontic failure at the first stage made it impossible to control the inflammation locally, whether by retreatment, apicectomy, or even extraction, and surgical debridement under general anesthesia was therefore inevitable. This case well demonstrates the importance of appropriate and sufficient root canal treatments during the first stage of the procedure.
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The photos used in this case report are published within the report.
RS, KT, and HH were responsible for writing and critical reviewing, and performed the final revision of the article. YO and HT performed the operation and have been following this patient. KK and TI diagnosed pathologically and contributed histopathological interpretation. TY and AE performed a critical review of the paper from an endodontic aspect. All the authors read and approved the final manuscript.
Ethics approval and consent to participate
Ethical approval was granted by the institutional review board at the Dental Hospital of the Tokyo Medical and Dental University (No. D2015–600).
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Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
The authors declare that they have no competing interests.
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