Soy isoflavones inducing overt hypothyroidism in a patient with chronic lymphocytic thyroiditis: a case report
Journal of Medical Case Reports volume 11, Article number: 253 (2017)
Many people have thyroid conditions that make them susceptible to hypothyroidism. If the foods they eat may interfere with the production of thyroid hormone, which can lead to development of serious hypothyroidism. The danger of health drinks should always be noted.
A 72-year-old Japanese woman was previously diagnosed with chronic lymphocytic thyroiditis caused by a goiter and had an elevated thyroid-stimulating hormone level (6.56 μIU/ml), a high anti-thyroid peroxidase antibody level (>600 IU/ml), and a high antithyroglobulin level (> 4000 IU/ml) but normal levels of free triiodothyronine (3.08 pg/ml) and thyroxine (1.18 ng/ml). She presented to our hospital with sudden-onset general malaise, edema, and hoarseness with an elevated thyroid-stimulating hormone (373.3 μIU/ml) level and very low triiodothyronine (< 0.26 pg/ml) and thyroxine (0.10 ng/ml) levels. It was determined that for 6 months she had been consuming a processed, solved health drink (“barley young leaf”) in amounts of 9 g/day, which included soybean and kale powder extract. Hypothyroidism might be affected by ingredients of health drinks. She discontinued consumption of the health drink immediately and began taking 12.5 μg of levothyroxine. The amount of levothyroxine was gradually increased every 3 days up to 100 μg. At day 61, her thyroid-stimulating hormone level had decreased (6.12 μIU/ml), her free triiodothyronine (2.69 pg/ml) and thyroxine (1.56 ng/ml) levels had increased, and her general condition was improved. Among risky foods lowering thyroid function, some experimental studies have revealed that isoflavones reduce thyroid function. Therefore, we measured the presence of isoflavones in the patient’s frozen serum with thin-layer chromatography. After she discontinued consumption of the health drink, two components quickly disappeared, and the other three components gradually decreased. On the basis of developing solvent composition and a positive ferric chloride reaction in thin-layer chromatography experiment, the five ingredients that disappeared or decreased were highly suspected to be soy isoflavones.
This case emphasizes that consuming health drinks that include soy isoflavone powder extracts can lead to severe hypothyroidism.
Many people have thyroid conditions that make them susceptible to hypothyroidism. For example, 10% of a disease-free population was reported to have positive results for anti-thyroid peroxidase antibodies (TPOAb) or antithyroglobulin (anti-Tg) , indicating that many patients have potentially chronic lymphocytic thyroiditis. However, there are many exogenous foods influencing the thyroid. If the foods interfere with the production of thyroid hormone, they can cause serious hypothyroidism.
We report a case of a patient with severe hypothyroidism induced by consuming a health drink. To the best of our knowledge, there are almost no other reports of patients with severe hypothyroidism induced by isoflavone. The components of the substances suspected to be isoflavones disappeared or decreased along with the clinical impairment of our patient. To the best of our knowledge, this is the first report of the presence of isoflavone in the serum of a patient with severe hypothyroidism.
A 72-year-old Japanese woman had been followed because of chronic lymphocytic thyroiditis caused by a goiter. She had an elevated thyroid-stimulating hormone (TSH) level (6.56 μIU/ml), a high TPOAb level (> 600 IU/ml), and a high anti-Tg level (> 4000 IU/ml) but normal levels of free triiodothyronine (T3; 3.08 pg/ml) and thyroxine (T4; 1.18 ng/ml). Her past medical history included only hypertension. She did not have any fever or neck pain suggestive of subacute or painless thyroiditis during recent months, and she had not taken any medication that would reduce thyroid function. Her social, family, and environmental histories were also unrevealing.
The patient had been taking a processed, solved health drink (“barley young leaf”) in amounts of 9 g/day, which included soybean and kale powder extract, for 6 months. She had gradually developed general malaise, edema, and hoarseness. She presented to our hospital with sudden-onset elevated TSH (373.3 μIU/ml) level and very low T3 (< 0.26 pg/ml) and T4 (0.10 ng/ml) levels. Her levels of total cholesterol, triglyceride, lactate dehydrogenase, aspartate transaminase, creatine phosphokinase, and immunoglobulin G were increased. These laboratory findings are shown in Table 1. Her goiter was swollen with mild hardness; her blood pressure at admission was 123/77 mmHg; her body temperature was 36.0 °C; and her pulse rate was 55 beats/minute. Computed tomography (Fig. 1) and thyroid ultrasound revealed bilateral thyroid enlargement. In addition, her uptake rate of 99mTc-pertechnetate was reduced. On the basis of these findings, we diagnosed severe hypothyroidism affected by ingredients of the health drink. She discontinued consumption of the health drink immediately and began taking 12.5 μg of levothyroxine. The amount of levothyroxine was gradually increased every 3 days up to 100 μg. After oral administration of levothyroxine, her high TSH levels decreased gradually, and her low free T3 and T4 levels increased. Her swelling and hoarseness disappeared, and her general condition improved. Her free T3 and T4 levels did not decrease in the follow-up period of the subsequent 6 months. The time line of this patient’s clinical course is shown in Fig. 2. Her TSH, free T3, and T4 levels are shown in Table 2.
There are many risky foods lowering thyroid function, such as soybean and cruciferous vegetables, especially in a patient with chronic lymphocytic thyroiditis. Among these foods, some experimental studies have shown that isoflavone reduces thyroid function [2, 3]. It was suspected that isoflavones might be the reason for our patient’s hypothyroidism. Therefore, we measured the presence of isoflavone in the patient’s frozen serum at 5 points from before admission to day 102 for further investigation. A blood sample was pretreated as described previously . The sample was analyzed with thin-layer chromatography on precoated silica gel 60 F254 or RP-18 WF254 plates (Merck Millipore Corporation, Darmstadt, Germany), with detection achieved by spraying with iron(III) chloride solution. After she discontinued consuming the health drink, two components quickly disappeared, and the other three components gradually decreased (Fig. 3). On the basis of developing solvent composition and a positive ferric chloride reaction in thin-layer chromatography experiment, the five ingredients that disappeared or decreased were highly suspected to be soy isoflavones.
Isoflavone, one of the isoflavonoids, is associated with breast cancer, prostate cancer, cerebral infarction, and myocardial infarction [5,6,7]. Moreover, some researchers have reported that soy isoflavone might reduce thyroid function by suppressing thyroid peroxidase [2, 3]. In our patient, the components of the substances suspected to be isoflavones had disappeared or decreased along with her clinical impairment. To the best of our knowledge, this is the first case report in which the presence of isoflavone has been examined in the serum of a patient with severe hypothyroidism. This point is unique; to the best of our knowledge, there have been almost no other reports in which the presence of isoflavone has been examined in patient serum. The other following two mechanisms of hypothyroidism by soybean are considered: (1) An alcohol-soluble component in soybean inhibits iodide uptake , and (2) phytic acid salt in soybean chelates the essential minerals, especially zinc, for thyroid hormone production [9, 10]. Our patient presented with severe hypothyroidism after 6 months of regular consumption of a health drink. Because the thyroid contains several months’ storage of thyroid hormone , overt hypothyroidism might present late. It is necessary to investigate further the detailed mechanisms of hypothyroidism caused by soy isoflavones.
This case emphasizes that consuming health drinks that include soy isoflavone powder extract can lead to severe hypothyroidism.
Hollowell JG, Staehling NW, Flanders WD, Hannon WH, Gunter EW, Spencer CA, et al. Serum TSH, T4, and thyroid antibodies in the United States population (1988 to 1994): National Health and Nutrition Examination Survey (NHANES III). J Clin Endocrinol Metab. 2002;87:489–99.
Chang HC, Doerge DR. Dietary genistein inactivates rat thyroid peroxidase in vivo without an apparent hypothyroid effect. Toxicol Appl Pharmacol. 2000;168:244–52.
Munro IC, Harwood M, Hlywka JJ, Stephen AM, Doull J, Flamm WG, et al. Soy isoflavones: a safety review. Nutr Rev. 2003;61:1–33.
Kaneko H, Yamato Y, Teramura Y, Fujiwara T, Suzuki A, Kawamura A, et al. Metabolites of micafungin in rats and dogs. Jpn J Chemother. 2002;50:88–93.
Yamamoto S, Sobue T, Kobayashi M, Sasaki S, Tsugane S. Japan Public Health Center-Based Prospective Study on Cancer Cardiovascular Diseases Group. Soy, isoflavones, and breast cancer risk in Japan. J Natl Cancer Inst. 2003;95:906–13.
Kokubo Y, Iso H, Ishihara J, Okada K, Inoue M, Tsugane S, JPHC Study Group. Association of dietary intake of soy, beans, and isoflavones with risk of cerebral and myocardial infarctions in Japanese populations: the Japan Public Health Center-based (JPHC) study cohort I. Circulation. 2007;116:2553–62.
Kurahashi N, Iwasaki M, Sasazuki S, Otani T, Inoue M, Tsugane S, Japan Public Health Center-Based Prospective Study Group. Soy product and isoflavone consumption in relation to prostate cancer in Japanese men. Cancer Epidemiol Biomarkers Prev. 2007;16:538–45.
Tran L, Hammuda M, Wood C, Xiao CW. Soy extracts suppressed iodine uptake and stimulated the production of autoimmunogen in rat thyrocytes. Exp Biol Med (Maywood). 2013;238:623–30.
Zhou JR, Erdman Jr JW. Phytic acid in health and disease. Crit Rev Food Sci Nutr. 1995;35:495–508.
Szkudelski T. Phytic acid-induced metabolic changes in the rat. J Anim Physiol Anim Nutr (Berl). 2005;89:397–402.
Ingbar SH, Woeber KA. The thyroid gland. In: Williams RH, editor. Textbook of endocrinology. 6th ed. New York: Saunders; 1981. p. 117–247.
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Nakamura, Y., Ohsawa, I., Goto, Y. et al. Soy isoflavones inducing overt hypothyroidism in a patient with chronic lymphocytic thyroiditis: a case report. J Med Case Reports 11, 253 (2017). https://doi.org/10.1186/s13256-017-1418-9