Pancreatic metastases from ocular malignant melanoma: the use of endoscopic ultrasound-guided fine-needle aspiration to establish a definitive cytologic diagnosis: a case report
Journal of Medical Case Reports volume 10, Article number: 332 (2016)
When encountering solid pancreatic lesions, nonpancreatic primary metastases are rare and differentiating a metastasis from a primary neoplastic lesion is challenging. The clinical presentation and radiologic features can be similar and the possibility of a pancreatic metastasis should be considered when the patient refers to a history of a different primary cancer. Endoscopic ultrasound offers a key anatomical advantage in accessing the pancreas and endoscopic ultrasound-guided fine-needle aspiration has become the gold standard method for diagnosing pancreatic lesions.
A 58-year-old white Hispanic woman with a history of uveal malignant melanoma, presented with abdominal pain and jaundice. On admission, laboratory tests were performed (her total bilirubin was 6.37 mg/dL with a direct fraction of 5.30 mg/dL). Cross-sectional, abdominal computed tomography with contrast, showed a low-attenuating lesion localized in the pancreatic head (measuring 4 × 3 cm) and a thinner section of the distal bile duct suspicious for compression. Our patient was scheduled for an endoscopic ultrasound-guided fine-needle aspiration to establish a diagnosis. Endoscopic ultrasound showed a solid, hypoechoic, well-defined lesion with regular contours (measuring 3.17 × 2.61 cm), localized between the head and neck of the pancreas. Endoscopic ultrasound-guided fine-needle aspiration was performed with a 22G needle and cytology confirmed the diagnosis of metastatic melanoma. Our patient subsequently underwent right orbital exenteration, followed by duodenopancreatectomy without complications. At the moment our patient is receiving adjuvant chemotherapy at an outside oncology clinic.
To the best of our knowledge, this is a very rare presentation of an ocular malignant melanoma with an isolated pancreatic metastasis causing symptomatic biliary obstruction. Endoscopic ultrasound-guided fine-needle aspiration has proven to be the best method to diagnose solid pancreatic lesions. In this particular case, cytology was essential in confirming the diagnosis and guiding the most adequate therapy, which was a pancreatic resection, ocular exenteration of the melanoma, followed by adjuvant chemotherapy.
Malignant pancreatic lesions generally arise from the gland itself. Nonpancreatic primary metastases are rare but usually seen in patients with widely metastatic disease . The frequency of this finding is variable, from 4.5 % in case series up to 11 % in data from autopsies. Isolated pancreatic metastasis can be found in only 2 % of cases [2–4].
The most common malignancies that metastasize to the pancreas are: renal, lung, breast, and colon cancer, followed by sarcoma and melanoma [5, 6]. Malignant melanoma commonly metastasizes to the gastrointestinal tract. Interestingly, autopsy-derived data demonstrates that 50 to 60 % of metastatic malignant melanoma patients can have gastrointestinal metastases but the clinical diagnosis is made only in 1.5 to 4.4 % of patients .
Metastatic malignant melanoma usually affects multiple sites. Isolated organ metastases are unusual and isolated pancreatic metastases are extremely rare. Uveal malignant melanoma is the most common primary intraocular malignancy. The majority of patients present with visual complaints, but as part of their primary workup, abdominal cross-sectional imaging should be performed since the primary metastatic location is the liver, in over 90 % of cases . We found only one case report of a patient with metastatic uveal melanoma where the lesion spread to the gallbladder and head of the pancreas .
Endoscopic ultrasound (EUS) provides us with high-quality images to look at the pancreas and nearby structures and the linear array echo endoscope has a working channel that allows us to accurately target different areas of the pancreas with minimal trauma to surrounding tissues. For this reason, endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) is now the gold standard method for sampling the pancreas. In the particular case of pancreatic metastasis, EUS-FNA has shown to be very useful, since establishing a diagnosis with cross-sectional imaging alone is not possible and this method offers the advantage of real-time tissue acquisition for diagnostic confirmation. This is of particular importance for identifying adequate surgical candidates, for avoiding unnecessary surgeries, and helping triage nonsurgical patients for less invasive procedures [10, 11].
Since this is a rare finding, there is a scarce amount of data addressing the surgical management of these pancreatic metastases. Patients who have isolated nonpancreatic primary metastases have shown to have excellent surgical outcomes and long-term survival can be achieved in patients who have these lesions resected [4, 12, 13].
This is the case of a 58-year-old white Hispanic woman with a history of uveal melanoma in her right eye (Fig. 1). She was admitted to the hospital with jaundice and abdominal pain for 10 days. On admission, laboratory tests were obtained (a complete blood count was within normal limits, amylase: 136 U/L, total bilirubin: 6.37 mg/dL with a direct fraction of 5.30 mg/dL). Cross-sectional, abdominal computed tomography (CT) with contrast, showed a low-attenuating lesion localized in the pancreatic head (measuring 4 × 3 cm) and a thinner section of the distal bile duct suspicious for compression).
After a multidisciplinary meeting, our patient was scheduled for EUS-FNA. EUS showed a solid, heteroechoic with predominantly hypoechoic areas, well-defined lesion with regular contours (measuring 3.1 × 2.6 cm), localized between the head and neck of the pancreas (Fig. 2). There was no vascular or lymph node invasion identified. EUS-FNA was performed with a 22G needle using the fanning technique.
In the cytology specimens (Fig. 3), the cells presented have a discohesive dispersed pattern, with marked variation in size and shape including epithelioid and spindle-shaped cells with plasmacytoid and round nuclei. The nuclear chromatin is clumping and irregular with excessive parachromatin clearing, there are single or multiple macronucleoli, and abundant cytoplasm with deep brownish-black granules obscuring the cell details. The background shows necrosis and hemorrhage.
Our patient underwent right orbital exenteration and followed by duodenopancreatectomy (the Whipple procedure), without any complications (Fig. 4). The surgical specimen showed undifferentiated cells, with extended disposition of brown pigment, infiltrating the pancreas and the serosa of the duodenal wall. Immunohistochemistry was positive for Melan-A, HMB45, vimentin, S-100 protein and negative for cytokeratin, all consistent with metastatic malignant melanoma. At the moment, our patient is receiving adjuvant chemotherapy at an outside oncology clinic.
Most of the solid pancreatic tumors are primary in origin and nonprimary pancreatic metastases are very rare , with just a few reported cases in the literature. The most common primary locations are kidney, lung, and breast [5, 6]. In this particular case, we identified a patient with a previous uveal melanoma that had an isolated pancreatic metastasis.
In general, over 50 % of patients with pancreatic metastases are discovered incidentally during imaging studies, only the minority of patients with pancreatic metastases present with symptoms of malignant biliary obstruction (jaundice, abdominal pain, and weight loss) making this a challenging clinical scenario [14, 15].
When evaluating solid pancreatic lesions, computed tomography (CT), magnetic resonance imaging (MRI), and EUS are very sensitive diagnostic methods, however, EUS allows for sampling, making this the preferred method to secure a diagnosis .
Studies have reported a sensitivity of 87–100 % using endoscopic ultrasound in detecting solid pancreatic lesions. Also, provides a high negative predictive value in ruling out pancreatic masses in the case these are not identified by EUS. . When compared to CT, MRI and EUS have a better performance in detecting small lesions, especially if they are less than 3 mm in diameter both within the pancreas and in the duodenal wall .
To be able to distinguish a primary pancreatic tumor from a nonpancreatic metastasis, pancreatic sampling is critical. Aspirates of melanoma are cellular and consist of discohesive malignant-appearing cells with nuclear pleomorphism and prominent nucleoli. The admixture of malignant epithelioid and spindle-shaped cells and the presence of melanin pigment are helpful clues to establishing the diagnosis. Confirmatory immunostaining was demonstrating immunoreactivity for some or all of melanoma markers, such as S100, HMB-45, Melan-A and micropthalmia transcription factor (MITF), can be used to aid the diagnosis of metastatic melanoma .
This case report illustrates an interesting and rare clinical scenario of nonpancreatic primary metastases from uveal malignant melanoma causing malignant biliary obstruction. That was conclusively proven using EUS-FNA.
Uveal malignant melanoma is the most common intraocular malignancy and it commonly metastasizes to the liver, in over 90 % of cases. This is a rare case report of an isolated nonpancreatic primary metastases. EUS-FNA is the gold standard method to characterize and diagnose solid pancreatic lesions, making this an invaluable tool to help guide the clinical management and decide a patient’s surgical candidacy. In carefully selected cases, the resection of isolated nonpancreatic primary metastases has shown to have good outcomes and improve the survival of these patients.
endoscopic ultrasound-guided fine-needle aspiration
magnetic resonance imaging
Eloubeidi MA, Tamhane AR, Buxbaum JL. Unusual, metastatic, or neuroendocrine tumor of the pancreas: a diagnosis with endoscopic ultrasound-guided fine-needle aspiration and immunohistochemistry. Saudi J Gastroenterol. 2012;18:99–105.
Waters L, Si Q, Caraway N, Mody D, Staerkel G, Sneige N. Secondary tumors of the pancreas diagnosed by eundoscopic ultrasound-guided fine-needle aspiration: a 10-year experience. Diagn Cytopathol. 2014;42(9):738–43.
Layfield LJ, Hirschowitz SL, Adler DG. Metastatic disease to the pancreas documented by endoscopic ultrasound guided fine-needle aspiration: a seven- year experience. Diagn Cytopathol. 2012;40(3):228–33.
Sperti C, Pasquali C, Liessi G, et al. Pancreatic resection for metastatic tumors to the pancreas. J Surg Oncol. 2003;83(3):161–6.
Goyal J, Lipson EJ, Rezaee N, et al. Surgical resection of malignant melanoma metastatic to the pancreas: case series and review of literature. J Gastrointest Cancer. 2012;43:431–6.
Fusaroli P, d’Ercole MC, De Giorgio R, et al. Contrast harmonic endoscopic ultrasonography in the characterization of pancreatic metastases (with video). Pancreas. 2014;43:584–7.
McLoughlin JM, Zager JS, Sondak VK, Berk LB. Treatment options for limited or symptomatic metastatic melanoma. Cancer Control. 2008;15(3):239–47.
Chattopadhyay C, Kim DW, Gombos DS, Oba J, Qin Y, Williams MD, Esmaeli B, Grimm EA, Wargo JA, Woodman SE, Patel SP. Uveal melanoma: from diagnosis to treatment and the science in between. Cancer. 2016;122(15):2299–312.
Cunningham JD, Cirincione E, Ryan A, Canin-Endres J, Brower S. Indications for surgical resection of metastatic ocular melanoma. A case report and review of the literature. Int J Pancreatol. 1998;24(1):49–53.
Pang JC, Roh MH. Metastases to the pancreas encountered on endoscopic ultrasound-guided, fine-needle aspiration. Arch Pathol Lab Med. 2015;139(10):1248–52.
DeWitt J, Jowell P, Leblanc J, et al. EUS-guided FNA of pancreatic metastases: a multicenter experience. Gastrointest Endosc. 2005;61:689–96.
Crippa S, Angelini C, Mussi C, et al. Surgical treatment of metastatic tumors to the pancreas: a single center experience and review of the literature. World J Surg. 2006;30(8):1536–42.
Reddy S, Edil BH, Cameron JL, Pawlik TM, Herman JM, Gilson MM, Campbell KA, Schulick RD, Ahuja N, Wolfgang CL. Pancreatic resection of isolated metastases from nonpancreatic primary cancers. Ann Surg Oncol. 2008;15(11):3199–206.
Triantopoulou C, Kolliakou E, Karoumpalis I, et al. Metastatic disease to the pancreas: an imaging challenge. Insights Imaging. 2012;3:165–72.
Scatarige JC, Horton KM, Sheth S, et al. Pancreatic parenchymal metastases: observations on helical CT. AJR Am J Roentgenol. 2001;176:695–9.
Coronel E, Waxman I. State-of-the-art endoscopic procedures for pancreatic cancer. Future Oncol. 2016;12(17):2037–47.
Leung D, Schwartz L. Imaging of neuroendocrine tumors. Semin Oncol. 2013;40(1):109–19.
Gornals J, Varas M, Catalá I, et al. Definitive diagnosis of neuroendocrine tumors using fine needle aspiration-puncture guided by endoscopic ultrasonography. Rev Esp Enferm Dig. 2011;103:123–8.
Bernacki KD, Betz BL, Weigelin HC, et al. Molecular diagnostics of melanoma fine-needle aspirates: a cytology-histology correlation study. Am J Clin Pathol. 2012;138(5):670–7.
We thank the patient and her family for her trust and cooperation for this case report.
Availability of data and materials
Endoscopic Ultrasound Aspiration Needle: 22 g needle. Expect™ Slimline (Boston Scientific, Marlborough, MA, USA).
DTHDM, MC and EGHDM wrote the manuscript. DAC and RT performed the histological examination. SC and ÉLAA performed the EUS-FNA. JJ is the surgeon of our team. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
Ethics approval and consent to participate
The ethics committee of the “Hospital das Clinicas da Facultade de Medicina da USP”, for the research protocol under the name of EUS vs ERCP for the anatomy-pathologic diagnoses of malignant biliary stenosis: a prospective study, approved the study with the reference number 1.481.669. CAAE #: 54011816.6.0000.0068. Ethics approval, consent and permission were giving for this study. Consent to publish was obtained from the participant to report any individual data, including any tissue samples, images, videos or voice recording.
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De Moura, D.T.H., Chacon, D.A., Tanigawa, R. et al. Pancreatic metastases from ocular malignant melanoma: the use of endoscopic ultrasound-guided fine-needle aspiration to establish a definitive cytologic diagnosis: a case report. J Med Case Reports 10, 332 (2016). https://doi.org/10.1186/s13256-016-1121-2