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Cushing syndrome as presenting symptom of calcifying nested stromal–epithelial tumor of the liver in an adolescent boy: a case report
© The Author(s). 2016
Received: 26 January 2016
Accepted: 12 May 2016
Published: 16 June 2016
Ectopic adrenocorticotropic hormone-producing primary liver tumors are rare, especially in children. We report the case of an adolescent boy of mixed Dutch and Moroccan descent with an adrenocorticotropic hormone-producing calcifying nested stromal–epithelial tumor with long-term follow-up. Thus far, only two such cases have been reported.
A 16-year-old boy of mixed Dutch and Moroccan descent presented with Cushing syndrome and a palpable abdominal mass. A calcifying nested stromal–epithelial tumor was diagnosed. Postoperatively, his plasma adrenocorticotropic hormone concentration normalized. He remains in complete remission 13 years after tumor resection.
Calcifying nested stromal–epithelial tumor should be in the differential diagnosis of liver tumors, especially if associated with Cushing syndrome as significant morbidity and mortality may be associated. Literature on the topics involved is comprehensively reviewed.
Calcifying nested stromal–epithelial tumor (CNSET) of the liver is a rare tumor consisting of nests of spindled epithelioid cells with a potential for calcification . Since its description, 37 cases have been reported (reviewed in [1–8]). Three small series of four, six and nine cases respectively, were reported as “Desmoplastic nested spindle cell tumor of the liver”  or as (calcifying) nested stromal cell tumor: (C)NSET [1, 5]. A similar tumor was reported as “Ossifying malignant mixed epithelial and stromal tumor of the liver” . Patients’ age at diagnosis ranged from 1 to 34 years. Nine cases of CNSET with ectopic adrenocorticotropic hormone (ACTH) production have been described (reviewed in ), with six cases in adolescent females. Ectopic ACTH production is reported sporadically in liver tumors, for instance in hepatoblastoma , and combined hepatocellular carcinoma (HCC) and carcinoid . In this case report a 16-year-old boy with Cushing syndrome due to CNSET is presented; it is the third report of such a case in an adolescent male with the longest follow-up (13 years). After tumor resection, his Cushing syndrome disappeared.
Core needle biopsy showed irregular fields of epithelial cell nests in a desmoplastic stroma, and dissociation of cells, yielding to pseudovascular spaces. Cells varied from epithelioid to spindle shaped. Immunohistochemistry was positive for cytokeratin 19, anticytokeratin, neural cell adhesion molecule, smooth muscle actin (SMA) and ACTH. No mitoses and no distinctive cellular atypia were found. Stromal cells were SMA-positive. A small cell desmoplastic tumor was considered but ruled out by negative reverse transcriptase polymerase chain reaction for the Ewing sarcoma-related gene fusion product and Wilms’ tumor-related gene . Low-grade HCC with neuroendocrine features, potentially a variant of fibrolamellar HCC, was suggested. However, the spindle-shaped epithelial cells and the lack of eosinophilic cells did not fit with that.
We report the case of an adolescent patient with Cushing syndrome due to an ACTH-producing CNSET of the liver. While incidental cases such as the aforementioned hormone-producing hepatoblastoma (suggesting neuroendocrine differentiation) , and combined HCC and carcinoid  have been reported, overall, liver tumors producing hormones are rare. The differential diagnosis includes carcinoids, combined or coexisting HCC and neuroendocrine carcinoma, metastatic neuroendocrine tumor, metastatic primitive gonadal stromal tumor , and cystadenomas/cystadenocarcinomas with ovarian-like stroma . These latter tumors express alpha-inhibin and are reactive to estrogen and progesterone receptors suggesting a common early fetal origin of the cystadeno(carcino)ma and ovarian-like stroma . A special property of the liver tumor in our patient is that it produced a polypeptide hormone (ACTH). ACTH-producing tumors are more common in the thymus, thyroid, bronchus, lung, adrenals, and pancreas than in the liver [13, 14]. Localizations often suggest a neural crest origin . These tumors may also produce insulin and catecholamines. Most likely, the CNSET in our patient belonged to this group.
Cushing syndrome has an annual incidence of two to five cases per million, of which children make up 10 % . Ectopic ACTH-producing tumors account for 1 % of Cushing syndrome causes in adolescents . Given the low incidence of primary hepatic ACTH-producing tumors, liver locations of ACTH-producing tumors are more likely to represent metastases from neuroendocrine tumors originating in other abdominal organs than primary tumors, rendering primary liver tumors causing Cushing syndrome as reported here extremely rare.
Surgical resection is the treatment of choice . Cushing syndrome dissolves after complete resection of the causative tumor . Liver transplant has been incidentally reported in the treatment of CNSET; two patients died of postoperative complications and consecutive lung metastases, respectively; another was without evidence of disease at 2-year follow-up [4–6]. The role of chemotherapy is unclear . Histologic and immunological features of CNSET suggest a potential role for treatment directed at neuroendocrine tumors. A recent study shows altered mesenchymal–epithelial transition with deletions in the beta-catenin gene, suggesting another potential targeting route .
In general, in ectopic ACTH syndrome, histology of the causative tumor, dissemination of disease and control of hypercortisolemia influence morbidity and mortality . In a series of 43 patients with ectopic ACTH-induced Cushing syndrome, median survival was 32 months with overall mortality rate of 63 % . Progression of the causative malignancy and systemic infection were the leading causes of death .
CNSET has been suggested to have low malignant potential [1, 5, 7]. In that perspective, the level of aggression in treatment warranted is unclear. However, local recurrence after resection and re-recurrence after treatment of recurrence with radiofrequency ablation have been described [1–5]. Recently, extrahepatic lymph node and lung metastases were reported [4, 6]. However, the patient we present here is alive and well 13 years after complete resection. With few reported cases, the biological behavior of CNSET is difficult to predict.
Larger series through international collaboration such as Children’s Hepatic tumors International Collaboration (CHIC) of the Société Internationale d’Oncologie Pédiatrique – Epithelial Liver Tumor Study Group (SIOPEL), are needed before conclusions about epidemiology, treatment, and prognosis can be drawn.
Despite its rarity, CNSET should be in the differential diagnosis of liver tumors, especially if associated with Cushing syndrome, as significant morbidity and mortality may be associated.
(C)NSET, (calcifying) nested stromal cell tumor; ACTH, adrenocorticotropic hormone; CHIC, Children’s Hepatic tumor International Collaboration; CNSET, calcifying nested stromal–epithelial tumor; HCC, hepatocellular carcinoma; MRI, magnetic resonance imaging; SIOPEL, Société Internationale d’Oncologie Pédiatrique – Epithelial Liver Tumor Study Group; SMA, smooth muscle actin
VBW acquired data, drafted, and revised the manuscript; PhR acquired data and was involved in drafting part of the manuscript; HB acquired data and was involved in drafting part of the manuscript; JZ acquired data and revised the manuscript; WHL was involved in conception and interpretation of data and revised the manuscript; DCA was involved in conception and revised the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Written informed consent was obtained from the patient’s legal guardian for publication of this case report and accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
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