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Postsurgical Pantoea calida meningitis: a case report

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Abstract

Introduction

Pantoea calida, a recently described environmental Enterobacteriaceae organism, has not yet been associated with human infection.

Case presentation

We report a case of postoperative meningitis caused by P. calida. After pituitary adenoma resection, a 52-year-old Caucasian woman developed febrile meningitis confirmed by cerebrospinal fluid analysis. P. calida was grown in pure culture from this fluid and was firmly identified with partial rpoB gene sequencing. She was cured by a 14-day course of meropenem.

Conclusions

P. calida must be added to the list of opportunistic Enterobacteriaceae pathogens responsible for postsurgical meningitis. It is easily identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Introduction

Pantoea calida has been recently described as a species of Enterobacteriaceae after its seminal isolation and culture from powdered infant formula [1]. It has not yet been associated with any infections and no clinical isolate has been reported. Here, we isolated one strain of P. calida from a cerebrospinal fluid (CSF) specimen and identified it as the cause of postsurgical meningitis.

Case presentation

A 52-year-old Caucasian woman had a medical history of hypertension, smoking and alcoholism. She presented with binocular diplopia. A computed tomography scan showed an intrasellar lesion with invasion of her right cavernous sinus that was further confirmed by brain magnetic resonance imaging. An endoscopic endonasal biopsy was performed and histopathology showed a nonspecific inflammation and subacute hemorrhagic alteration of her nasal mucosa. An antibiotic prophylaxis with cefazolin 2gr was given. A transnasal surgical excision was performed, and no leak of CSF was observed after the surgery. Histopathology confirmed a single prolactin pituitary adenoma. Five days after the surgery, she presented a fever (38.6°C) and a meningeal syndrome with consciousness disorder, neck stiffness and a Glasgow Coma Scale evaluated at 13 (E4V4M5) but without focal neurological deficits. The CSF collected by lumbar puncture showed hypoglycorrhachia (1.33mmol/L; CSF/serum glucose ratio=0.20), an elevated protein level (5.88g/L) and 4500 leukocytes/mL including 98% polymorphonuclear leukocytes. There was no detectable organism on direct microscopic examination after Gram staining. Treatment combining intravenous vancomycin 1gr twice a day and intravenous meropenem 2gr three times a day was started for presumptive postsurgical meningitis. Her clinical condition rapidly improved, with apyrexia and a net regression of the meningeal syndrome within 2 days. Five days after the onset of symptoms, vancomycin was stopped and meropenem 6gr per day was continued for a total of 14 days. At 90-day follow-up she was afebrile, healthy and without any sign of meningitis.

The CSF was inoculated on Polyvitex (bioMérieux, La Balme-les-Grottes, France) and Columbia agar with 5% sheep blood and incubated at 37°C under a 5% carbon dioxide atmosphere. After 48-hour incubation, small whitish round shiny domed colonies were observed on both culture media. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) performed as previously described [2, 3] yielded P. calida with identification score of 2.26, 2.17 and 2.17 for the three spots deposited on the MALDI-TOF-MS plate. Furthermore partial rpoB gene sequencing performed as previously reported [4] confirmed the identification of P. calida with 99.88% sequence similarity with the reference sequence (GenBank GQ892191). Antimicrobial susceptibility was performed by diffusion method in agar and incubated aerobically for 24 hours at 37°C. The isolate was resistant to ampicillin (minimal inhibitory concentration (MIC) >8mg/L), amoxicillin-clavulanate (MIC, 1mg/L), and cephalothin (MIC, 1mg/L). It was susceptible to ceftriaxone (MIC, 0.5mg/L), imipenem (MIC, 0.5mg/L), ciprofloxacin (MIC, 0.25mg/L), gentamicin (MIC<1mg/L) and co-trimoxazole (MIC<1mg/L).

Discussion

Here, P. calida was isolated from the CSF of a patient with postoperative meningitis [5]. The isolate was firmly identified by using two different complementary approaches: MALDI-TOF-MS which is an advanced proteomics method [2, 3] relying on the analysis of unique peptidic signatures; and partial rpoB gene sequencing which relies on the analysis of unique nucleotidic signatures [6]. These two technical approaches yielded unambiguous, concordant identification. In particular, MALDI-TOF-MS proved to be efficient to identify this species, despite the fact that no clinical isolate was previously included in the database.

The absence of any bacteria other than P. calida on the culture of CSF, as well as the fact that it was the first identification of this microorganism in our laboratory, secure the conclusion that P. calida was responsible for this postoperative meningitis. Accordingly, the patient was rapidly cured after an effective antibiotic treatment was administrated. In this case, the source of infection remained unknown. Indeed, P. calida has been documented only in powdered infant formula [1] and our patient had no contact with such formula. Of interest, the Cronobacter species (formerly Enterobacter sakazakii) causing health-care-associated meningitis in neonates [7] is also contaminating powdered infant formula [8, 9].

Indeed, P. calida is closely related to members of the Enterobacteriaceae genus Erwinia, Tatumella, Kluyvera, Citrobacter and Cronobacter, which also comprise opportunistic pathogens causing meningitis, mainly in newborns [1]. Whereas Erwinia and Tatumella organisms have not been associated with central nervous system infection, Kluyvera meningitis has been reported in a newborn [10]. Also Citrobacter bacteria are opportunistic pathogens seldom reported as causing meningitis in neonates [11], children [12] and adults with cancer [13]. Recently, one case of Citrobacter koseri meningitis has been reported after free-diving [14]. Lastly, Cronobacter species along with Enterobacter species have been reported as causes of health-care-associated meningitis [15].

Conclusions

P. calida should be added to the list of Enterobacteriaceae pathogens responsible for infectious postoperative meningitis. Sources different from powdered infant formula should be investigated. P. calida meningitis can be cured by appropriate antibiotic treatment. This new pathogen can easily be identified by using MALDI-TOF-MS [2, 3].

Consent

Written informed consent was obtained from the patient for publication of this case report and accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.

Abbreviations

CSF:

Cerebrospinal fluid

MALDI-TOF-MS:

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

MIC:

Minimal inhibitory concentration.

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Acknowledgements

This work was supported by URMITE, Marseille, France.

Author information

Correspondence to Michel Drancourt.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

NC, RN, SDLR, and PHR took care of the patient. SF and MD took care of the microbiology of the specimens. SF, NC, RN, and SDLR wrote the case. PHR and MD evaluated the draft and suggested revisions. All authors read and approved the final manuscript.

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Keywords

  • Health-care associated infection
  • Meningitis
  • Neurosurgery
  • Pantoea calida