Figure 2

Immunohistochemical evaluation of chemoattractants for macrophages. (a) Hematoxylin and eosin staining shows verruciform xanthoma consisting of hyperkeratosis, a few mitotic figures (arrows) and infiltrating foamy cells (asterisks, ×200). (b) Cytokeratin AE1/AE3 showing positive immunostaining in the epithelial cells of the verruciform xanthoma (×200). (c) CD68 showing positive cytoplasmic immunostaining exclusively in the majority of foam cells infiltrating beneath the basal cells (×200). (d) The parts densely stained for monocyte chemoattractant protein-1 (MCP-1) are observed in the differentiating keratinocytes over the basal and parabasal layers. Clusters of the infiltrating macrophages are also stained positive, albeit less intensely, for MCP-1 (arrows, ×200).