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Infective endocarditis with Lactococcus garvieae in Japan: a case report

  • Yukiko Watanabe1, 2,
  • Toshio Naito1, 2Email author,
  • Ken Kikuchi1, 2,
  • Yu Amari1,
  • Yuki Uehara1, 2,
  • Hiroshi Isonuma1,
  • Teruhiko Hisaoka1,
  • Terutoyo Yoshida4,
  • Kenji Yaginuma3,
  • Norihide Takaya3,
  • Hiroyuki Daida3 and
  • Keiichi Hiramatsu2
Journal of Medical Case Reports20115:356

https://doi.org/10.1186/1752-1947-5-356

Received: 13 December 2010

Accepted: 9 August 2011

Published: 9 August 2011

Abstract

Introduction

Lactococcus garvieae is a well-recognized fish pathogen, and it is considered a rare pathogen with low virulence in human infection. We describe the 11th case of L. garvieae infective endocarditis reported in the literature, and the first reported case in Japan.

Case presentation

We report a case of a 55-year-old Japanese woman who had native valve endocarditis with L. garvieae. The case was complicated by renal infarction, cerebral infarction, and mycotic aneurysms. After anti-microbial treatment, she was discharged from the hospital and is now well while being monitored in the out-patient clinic.

Conclusion

We encountered a case of L. garvieae endocarditis that occurred in a native valve of a healthy woman. The 16S ribosomal RNA gene sequencing was useful for the identification of this pathogen. Although infective endocarditis with L. garvieae is uncommon, it is possible to treat high virulence clinically.

Introduction

Lactococcus garvieae was first isolated from cases of bovine mastitis [1], and it is rarely reported in human infection. The first human infection was documented as infective endocarditis (IE) in 1991 [2]. Since then, it has been identified as a pathogen in IE [39], liver abscess [10], and osteomyelitis [4]. We describe the 11th case of L. garvieae IE reported in the literature, and the first reported case in Japan.

Case presentation

A 55-year-old healthy Japanese woman presented to our hospital with a two-month history of malaise and myalgia, and she had had a fever for one month. The patient had no significant medical history and no relevant history regarding pets, travel, dental treatment, or drug abuse. Her occupational history was that she worked at an import grocery store. During her physical examination, we noted her body temperature of 37.6°C, a grade 2 systolic murmur at the apex, and a painful 1 mm black induration on her right forefinger. Trans-thoracic echocardiography showed a 1 cm mobile mass on the mitral valve with moderate mitral regurgitation. Her laboratory tests showed a white blood cell count of 8200/μL, a C-reactive protein level of 6.6 mg/dL, and a hemoglobin level of 9.9 g/dL. Gram-positive cocci forming pairs or short chains were shown by Gram staining of three sets of blood cultures drawn at the time of admission.

IE was diagnosed on the basis of the Duke criteria for the diagnosis of infective endocarditis [11] (two major and two minor criteria) and antibiotic treatment with benzylpenicillin 3 million units every four hours and gentamicin 60 mg every eight hours was started, as Streptococcus sp. was suspected as the causative pathogen. Subsequently, the rapid ID32 Strep (bioMérieux, Marcy-l'Etoile, France) profile (code 34323501010) revealed L. garvieae. We also performed molecular species identification by 16S ribosomal RNA gene (16S rRNA) sequencing, and the almost full-length 16S rDNA matched L. garvieae (99.9%). The results of an anti-microbial susceptibility test using Etest strips (AB Biodisk, Dalvagen, Solna, Sweden) showed the following values: erythromycin 0.25 mg/L, clindamycin ≥ 256 mg/L, vancomycin 0.38 mg/L, linezolid 2.0 mg/L, penicillin 0.5 mg/L, ceftriaxone 0.38 mg/L, gentamicin 1.5 mg/L, and streptomycin 64 mg/L. Considering these results, we changed the anti-microbial treatment to intravenous ceftriaxone 2 g every 12 hours and gentamicin 80 mg every eight hours intraveneously.

Meanwhile, her body temperature rose to 39°C with left lower back pain on her sixth day in the hospital. Left-sided renal infarction was diagnosed on the basis of contrast-enhanced computed tomography (CT) (Figure 1). On the 12th hospital day, cerebral infarction occurred, followed by a sudden onset of right-sided hemiparesis and slurred speech (National Institutes of Health Stroke Scale (NIHSS) score 12). Diffusion-weighted MRI showed a cerebral infarction of the middle cerebral artery area (Figure 2). Furthermore, cerebral mycotic aneurysms were detected by three-dimensional CT angiography on the 24th hospital day (Figure 3). The patient also developed aspiration pneumonia after the cerebral infarction complication.
Figure 1

Contrast-enhanced abdominal computed tomography. Yellow arrow indicates left renal infarction.

Figure 2

Diffusion-weighted MRI scan. Yellow arrow indicates the middle cerebral artery areas of high signal intensity.

Figure 3

Three-dimensional computed tomography angiography. The two yellow circles indicate cerebral mycotic aneurysms.

Anti-microbial treatment was completed in nine weeks, including the treatment for pneumonia. Several repeat blood cultures thereafter were all negative. The vegetation on the mitral valve was no longer detected by trans-esophageal echocardiography performed just before she was discharged from the hospital. We found by CT that her left-sided renal infarction had improved, and the consequences of her cerebral infarction had also improved with only right-sided muscular weakness (NIHSS score 2). She was discharged from the hospital, and she is now well while being monitored in the out-patient clinic.

Discussion

Genus Lactococcus was separated from genus Streptococcus in 1985 based on DNA-DNA relatedness and 16S rRNA sequencing data published by Ludwig et al.[12]. The genus Lactococcus was classified into eight species and sub-species by Facklam and Elliott in 1995 [13] and Pu et al. in 2002 [14]: L. lactis ssp. lactis, L. lactis ssp. cremoris, L. lactis ssp. hordniae, L. garvieae, L. piscium, L. plantarum, L. raffinolactis, and L. xyloses. Cases involving the L. garvieae pathogen in human infection are rarely reported [210]. The major clinical presentation is IE, which has been reported in 10 cases in the literature [29]. Five of ten cases involved native valves [3, 69], and four cases required surgery for valve replacement [3, 6, 8, 9]. In addition, two cases were complicated by cerebral infarction or hemorrhage [3, 9]. L. garvieae IE is uncommon; however, the virulence of L. garvieae might be underestimated. The treatment for L. garvieae IE is not standardized, because the exact criterion of the susceptibility test has not been established. In the published cases, penicillin combined with gentamicin, amoxicillin combined with netilmicin, ampicillin, ceftriaxone, and vancomycin were used as anti-microbial treatment. In our case, the results of the anti-microbial susceptibility test of the isolate using the Etest method was susceptibility to ceftriaxone (0.38 mg/L), erythromycin (0.25 mg/L), vancomycin (0.38 mg/L), and linezolid (2 mg/L), less susceptibility to penicillin (0.5 mg/L), and resistance to clindamycin (> 256 mg/L).

Elliott and Facklam [15] reported that clindamycin resistance is a feature of L. garvieae, and this feature can be used to distinguish it from other lactococci. To confirm the precise identification, various molecular techniques are available: whole-cell protein analysis, 16S rRNA gene sequencing, and sodA int (an internal fragment of the sodA gene encoding the manganese-dependent superoxide dismutase) gene sequencing.

L. garvieae has pathogenicity for several fish species, ruminants, and humans. Therefore, it could be a potentially zoonotic infection. Investigators in one recent report hypothesized that eating infected raw fish carrying L. garvieae caused its infection [7]. To investigate the relationship between our isolate and fish isolates, the immunological property was examined by using several anti-sera against L. garvieae that have previously been produced by fish in Japan. The results showed that our isolate did not have a capsule detected by any anti-capsule antibody for fish isolates. Pulsed-field gel electrophoresis also showed that the pattern of our isolate was quite different from that of fish-derived strains (data not shown). Vela et al.[16] found great phenotypic heterogeneity and genetic diversity among the L. garvieae isolates from fish, cows, water buffalo, and humans, with a generally good correlation between the phenotypic and genetic properties of L. garvieae. As the origin of the L. garvieae strain in our patient could not be clarified by the investigations used to detect Japanese fish pathogens, other causes of her infection, such as cow's milk or cheeses, are possible. To confirm the etiology of the transmission of L. garvieae, larger molecular epidemiological studies including investigating causes from the environment, animals, and humans must be carried out.

Conclusion

Herein we report the first case of L. garvieae IE in Japan. Molecular biological investigation techniques such as 16S ribosomal RNA gene sequencing are useful for the identification of L. garvieae. Although L. garvieae is considered a rare pathogen with low virulence in human infection, it could cause native valve endocarditis in healthy people. It might be necessary to review the precise estimation of the pathogenicity of L. garvieae.

Consent

Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.

Declarations

Acknowledgements

We thank Dr Shigeki Misawa, Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan, for phenotypic characterization of the bacterial isolate.

Authors’ Affiliations

(1)
Department of General Medicine, Juntendo University School of Medicine
(2)
Department of Infection Control Science, Juntendo University School of Medicine
(3)
Department of Cardiology, Juntendo University School of Medicine
(4)
Department of Fisheries, Faculty of Agriculture, Miyazaki University

References

  1. Collins MD, Farrow JAE, Phillips BA, Kandler O: Streptococcus garvieae sp. nov. and Streptococcus plantarum sp. nov. J Gen Microbiol. 1983, 129: 3427-3431.PubMedGoogle Scholar
  2. Furutan NP, Breiman RF, Fischer MA, Facklam RR: Lactococcus garvieae infections in humans: a cause of prosthetic valve endocarditis [abstract C297]. Proceedings of the 91st General Meeting of the American Society for Microbiology. 1991, Dallas: American Society of Microbiology, 109-Google Scholar
  3. Fefer JJ, Ratzan KR, Sharp SE, Saiz E: Lactococcus garvieae endocarditis: report of a case and review of the literature. Diagn Microbiol Infect Dis. 1998, 32: 127-130. 10.1016/S0732-8893(98)00065-0.View ArticlePubMedGoogle Scholar
  4. James PR, Hardman SM, Patterson DL: Osteomyelitis and possible endocarditis secondary to Lactococcus garvieae: a first case report. Postgrad Med J. 2000, 76: 301-303. 10.1136/pmj.76.895.301.View ArticlePubMedPubMed CentralGoogle Scholar
  5. Fihman V, Raskine L, Barrou Z, Kiffel C, Riahi J, Berçot B, Sanson-Le Pors MJ: Lactococcus garvieae endocarditis: identification by 16S rRNA and sodA sequence analysis. J Infect. 2006, 52: e3-e6. 10.1016/j.jinf.2005.04.021.View ArticlePubMedGoogle Scholar
  6. Vinh DC, Nichol KA, Rand F, Embil JM: Native-valve bacterial endocarditis caused by Lactococcus garvieae. Diagn Microbiol Infect Dis. 2006, 56: 91-94. 10.1016/j.diagmicrobio.2006.02.010.View ArticlePubMedGoogle Scholar
  7. Wang CY, Shie HS, Chen SC, Huang JP, Hsieh IC, Wen MS, Lin FC, Wu D: Lactococcus garvieae infections in humans: possible association with aquaculture outbreaks. Int J Clin Pract. 2007, 61: 68-73.View ArticlePubMedGoogle Scholar
  8. Yiu KH, Siu CW, To KK, Jim MH, Lee KL, Lau CP, Tse HF: A rare cause of infective endocarditis: Lactococcus garvieae. Int J Cardiol. 2007, 114: 286-287. 10.1016/j.ijcard.2005.11.092.View ArticlePubMedGoogle Scholar
  9. Li WK, Chen YS, Wann SR, Liu YC, Tsai HC: Lactococcus garvieae endocarditis with initial presentation of acute cerebral infarction in a healthy immunocompetent man. Intern Med. 2008, 47: 1143-1146. 10.2169/internalmedicine.47.0795.View ArticlePubMedGoogle Scholar
  10. Mofredj A, Baraka D, Kloeti G, Dumont JL: Lactococcus garvieae septicemia with liver abscess in an immunosuppressed patient. Am J Med. 2000, 109: 513-514. 10.1016/S0002-9343(00)00534-9.View ArticlePubMedGoogle Scholar
  11. Durack D, Lukes A, Bright D, Duke Endocarditis Service: New criteria for diagnosis of infective endocarditis: utilization of specific echocardiographic findings. Am J Med. 1994, 96: 200-209. 10.1016/0002-9343(94)90143-0.View ArticlePubMedGoogle Scholar
  12. Ludwig W, Seewaldt E, Kilpper-Bälz R, Schleifer KH, Magrum L, Woese CR, Fox GE, Stackebrandt E: The phylogenetic position of Streptococcus and Enterococcus. J Gen Microbiol. 1985, 131: 543-551.PubMedGoogle Scholar
  13. Facklam R, Elliott JA: Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev. 1995, 8: 479-495.PubMedPubMed CentralGoogle Scholar
  14. Pu ZY, Dobos M, Limsowtin GK, Powell IB: Integrated polymerase chain reaction-based procedures for the detection and identification of species and subspecies of the Gram-positive bacterial genus Lactococcus. J Appl Microbiol. 2002, 93: 353-361. 10.1046/j.1365-2672.2002.01688.x.View ArticlePubMedGoogle Scholar
  15. Elliott JA, Facklam RR: Antimicrobial susceptibilities of Lactococcus lactis and Lactococcus garvieae and a proposed method to discriminate between them. J Clin Microbiol. 1996, 34: 1296-1298.PubMedPubMed CentralGoogle Scholar
  16. Vela AI, Vázquez J, Gibello A, Blanco MM, Moreno MA, Liébana P, Albendea C, Alcalá B, Mendez A, Domínguez L, Fernández-Garayzábal JF: Phenotypic and genetic characterization of Lactococcus garvieae isolated in Spain from lactococcosis outbreaks and comparison with isolates of other countries and sources. J Clin Microbiol. 2000, 38: 3791-3795.PubMedPubMed CentralGoogle Scholar

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© Watanabe et al; licensee BioMed Central Ltd. 2011

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