- Case report
- Open Access
- Open Peer Review
Clinical and histologic features of botryoid odontogenic cyst: a case report
© Farina et al; licensee BioMed Central Ltd. 2010
- Received: 7 December 2009
- Accepted: 10 August 2010
- Published: 10 August 2010
The lateral periodontal cyst, as the name implies, occurs on a lateral periodontal location and is of developmental origin, arising from cystic degeneration of clear cells of the dental lamina. A botryoid odontogenic cyst is considered to be a rare multilocular variant of a lateral periodontal cyst.
We report the clinical and histopathologic features of a rare case of botryoid odontogenic cyst found in an edentulous area corresponding to the right lower canine of a 64-year-old African-American woman. A multilocular radiolucency was observed, and surgical removal of the lesion revealed a nodule of rubber-like consistency measuring about 1.5 cm in diameter. Cross-sectioning of the nodule showed that it consisted of various cystic compartments. Histologically, various voluminous periodic acid-Schiff-negative clear cells randomly distributed throughout the cystic epithelium were observed, as well as cell layers showing thickenings generally formed by oval, sometimes entangled plaques. The capsule consisted of fibrous connective tissue and showed rare and discrete foci of a perivascular mononuclear inflammatory infiltrate and reactive bone-tissue fragments. The final diagnosis was botryoid odontogenic cyst.
We provide data that allow the reader to establish the differences between botryoid odontogenic cyst, glandular odontogenic cyst, and lateral periodontal cyst, helping with the differential diagnosis. The reader will have the opportunity to review botryoid odontogenic cyst clinical and histopathologic features, including treatment.
- Clear Cell
- Fibrous Connective Tissue
- Odontogenic Cyst
- Cystic Epithelium
- Dental Lamina
Botryoid odontogenic cyst (BOC) was originally described in 1973 by Weathers and Waldron  as an intraosseous lesion characterized by a macroscopic and microscopic multilocular growth pattern, resembling a bunch of grapes (from the Greek word botrios). BOC is considered to be a variant of a lateral periodontal cyst . Greer and Johnson , reviewing 10 cases of BOC, observed that nine of the lesions were located in the mandible, mainly the anterior region, and one in the maxilla, also in the anterior region. Radiologically, eight cases were characterized by unilocular radiolucencies, and two, by multilocular radiolucencies. The mean age of the patients was 46 years. Three cases were recurrences, one occurring eight years, and two, 10 years after treatment. Histologically, non-keratinized squamous epithelium consisting of a few cell layers was observed, which showed plaque-like thickening in seven cases. Zones of clear cells scattered throughout the epithelium and containing periodic acid-Schiff (PAS)-positive material were observed in all 10 cases. In four cases, a discretely hyalinized zone underlying the basal epithelial layer was noted. All 10 lesions were characterized by the presence of multiple cystic compartments.
In a study evaluating 66 cases of BOC, Ramer and Valuri  found a slight predominance in women (53%) over men (47%), with 70% of the cases occurring in white individuals and 30%, in black individuals. Heikinheimo et al. reported a case of BOC with multiple recurrences that had occurred over a period of nine years, which led the authors to propose more-radical surgical intervention in cases of BOC. Manor et al. stated that BOC frequently shows a lobulated radiographic pattern similar to that of glandular odontogenic cysts and, therefore, the latter should be included in the differential diagnosis. However, these cysts are characterized by the presence of salivary gland-like structures in the lining epithelium, an uncommon histologic finding in BOC .
A 64-year-old African-American woman presented at our outpatient clinic with a two-year history of asymptomatic enlargement in the right anterior region of the edentulous mandible.
The case of BOC reported in the present study was characterized clinically by an asymptomatic nodule located in the right anterior region of the mandible, and radiographically, by a multilocular radiolucent lesion, reflecting the macroscopic aspect of the surgical specimen, a phenomenon not always observed in BOC because this type of cyst tends to present a unilocular radiographic aspect . Conversely, the multilocular radiographic aspect is very frequent in cases of glandular odontogenic cysts ; however, these cysts show glandular ductlike structures in the lining epithelium, not observed in this case.
In the present case, the microscopic observation of multiple cystic cavities lined with non-keratinized stratified pavement epithelium consisting of a few cell layers with focal entangled thickenings, the presence of voluminous clear cells in the epithelium, and a thin connective tissue capsule with a few inflammatory cells, combined with the clinical and radiographic findings, defined the diagnosis of BOC, as also reported by Falcone et al.. The fact that the clear cells present in the epithelium were not stained with PAS indicates that they did not contain glycogen or is just an example of the vagaries of histochemical staining procedures. Negative findings may well be due to tissue handling or other technical details. This finding is in contrast to the results of Greer and Johnson  and Gurol et al., who observed staining of these cells with PAS. Greer and Johnson  reported a similarity between these clear cells rich in glycogen, frequently observed in the epithelium of BOC, and dental lamina cells, suggesting that the dental lamina is one of the possible origins of BOC. However, these cells do not seem to exert any influence on the biologic behavior of the cyst. The diagnosis of BOC should not be discarded in cases of negative PAS staining when all other histologic features are present.
With respect to the similarity between BOC and lateral periodontal cysts, Machado de Souza et al. stated that BOC are distinguished from the latter by their larger size, which, associated with the multilocular characteristic of BOC, increases the possibility of recurrence because complete surgical removal becomes more difficult.
Üçok et al. reported that the risk of recurrence of BOC is similar to that of keratocystic odontogenic tumors, but that the former shows less aggressive behavior. According to Ramer and Valuri , BOC is not an aggressive lesion, and recurrences are the result of conservative surgical treatment (enucleation). The authors observed that 10 of 13 recurrences studied presented a multilocular radiographic aspect and suggested more-frequent postoperative follow-up visits in these cases.
The case reported here has been followed up for eight years. So far, no clinical or radiographic evidence characterizes recurrence of the lesion, indicating that the lesion was removed in toto. However, follow-up for a longer period is necessary to ensure the success of surgical treatment in this case of BOC.
Recurrences of multilocular radiolucent lesions of BOC are common. A non-conservative surgical removal is the only effective treatment for this kind of lesion.
Written informed consent was obtained from the patient for publication of this case report and accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
- Weathers DR, Waldron CA: Unusual multilocular cysts of the jaws (botryoid odontogenic cysts). Oral Surg Oral Med Oral Pathol. 1973, 36: 235-241. 10.1016/0030-4220(73)90244-2.View ArticlePubMedGoogle Scholar
- Uçok O, Yaman Z, Günhan O, Uçok C, Dogan N, Baykul T: Botryoid odontogenic cyst: report of a case with extensive epithelial proliferation. Int J Oral Maxillofac Surg. 2005, 34: 693-695. 10.1016/j.ijom.2005.01.005.View ArticlePubMedGoogle Scholar
- Greer RO, Johnson M: Botryoid odontogenic cyst: clinicopathologic analysis of ten cases with three recurrences. J Oral Maxillofac Surg. 1988, 46: 574-579. 10.1016/0278-2391(88)90147-4.View ArticlePubMedGoogle Scholar
- Ramer M, Valauri D: Multicystic lateral periodontal cyst and botryoid odontogenic cyst. Multifactorial analysis of previously unreported series and review of literature. N Y State Dent J. 2005, 71: 47-51.PubMedGoogle Scholar
- Heikinheimo K, Happonen RP, Forssell K, Kuusilehto A, Virtanen I: A botryoid odontogenic cyst with multiple recurrences. Int J Oral Maxillofac Surg. 1989, 18: 10-13. 10.1016/S0901-5027(89)80006-2.View ArticlePubMedGoogle Scholar
- Manor R, Anavi Y, Kaplan I, Calderon S: Radiological features of glandular odontogenic cyst. Dentomaxillofac Radiol. 2003, 32: 73-79. 10.1259/dmfr/22912856.View ArticlePubMedGoogle Scholar
- Slater LJ: Botryoid odontogenic cyst versus glandular odontogenic cyst. Int J Oral Maxillofac Surg. 2006, 35: 775-10.1016/j.ijom.2006.02.023.View ArticlePubMedGoogle Scholar
- Falcone F, Lazow SK, Solomon MP, Berger JR: The botryoid odontogenic cyst: case report and twenty-five year literature review. J N J Dent Assoc. 1995, 66: 15-18.PubMedGoogle Scholar
- Gurol M, Burkes EJ, Jacoway J: Botryoid odontogenic cyst: analysis of 33 cases. J Periodontol. 1995, 66: 1069-1073.View ArticlePubMedGoogle Scholar
- de Souza SO, Campos AC, Santiago JL, Jaeger RG, de Araújo VC: Botryoid odontogenic cyst: report of a case with clinical and histogenic considerations. Br J Oral Maxillofac Surg. 1990, 28: 275-276. 10.1016/0266-4356(90)90067-U.View ArticleGoogle Scholar
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