Fig. 1

Flow cytometry showing a population of atypical T-cells (48% of total cells, 76% of total T-cells), highlighted in cyan expressing CD3 and CD8, but lacking CD4, CD5, and CD57. Analysis of KIR expression reveals the cells to preferentially express CD158b isoform; CD158a and CD158e (not shown) were not expressed. Normal background CD4⁺ and CD8⁺ T-cells are colored in red and green, respectively (A). Monoclonal T-cell receptor gamma gene rearrangement detected by PCR and capillary electrophoresis showing a single prominent amplicon present in a minimal polyclonal background. X axis = amplicon size. Y axis = fluorescence (B)