Fig. 2
From: Neonatal interstitial lung disease in a girl with Jacobsen syndrome: a case report
A, B Hematoxylin–eosin–saffron (HES) stain from lung wedge biopsy shows thickened alveolar septae and alveoli with reduced septation. B Increased amount of mesenchymal interstitial cells with clear cytoplasm in alveolar septae. C Periodic acid–Schiff (PAS) stain highlights glycogen granules (arrows) in the interstitial cells. D–F Transmission electron microscopy shows thickened alveolar septae (al.sep). The capillaries (cp) show normal distribution near the alveolar lumen (al). The alveolar epithelial cells (al.ep) contain normal amounts of surfactant. The interstitial cells in the alveolar septae contain increased amounts of glycogen granules (asterisk). Original magnification ×100 (A), ×400 (B, C). Scale bar: 100 mm (A), 50 mm (B, C), 20 µm (D), 5 µm (E), and 1 µm (F)