Results of basophil activation tests. a Sugammadex, but not γ-cyclodextrin, induced CD203c upregulation in the sugammadex-allergic patient. A maximum of 5% CD203c-positive basophils after stimulation with the buffer solution (negative control) was accepted. Asterisks indicate that the CD203c positivity rate is above the threshold. Thresholds calculated by our previous study were used to determine positivity for sugammadex . b Upregulation of CD203c expression with and without wortmannin was compared. Wortmannin significantly suppressed sugammadex- and anti-IgE-induced CD203c upregulation. Conversely, suppression of CD203c upregulation by fMLP was limited. NC negative control.