Legend to symbols: ++ = high expression, + = moderate expression, 0 = sporadic expression, – = no expression.
Legend to Table 2: Immunohistochemical expression pattern of lymphocytic specific markers revealed more frequent expression of CD3 in myocardial than in skeletal muscle tissue. In addition, within the CD3 positive T-cells CD4 positive cells were higher expressed than CD8 positive cells indicating a majority of the T-cell-helper-phenotype (CD4+) than of the T-cell-regulatory-phenotype (CD8+). This was supported by the perimysial expression of CD38 – a marker for activated CD4+-T-cells; and the lack of CD25 – a marker for activated CD8+-T-cells. Finally, evidence for an activated innate immune system was provided by the specific expression of CD56 and T-cell intracellular antigen (specific marker for a subpopulation of natural killer cells). Overall, immunohistochemical expression analysis revealed a myocardial-associated lymphatic-disordered status in dermatomyositis.