Figure 6

Immunostaining for caveolin-1 in normal liver areas (a-c) and the lesion (d-f). For the lesion, immunostaining was performed on serial sections continuous with those used in Figure 4. a-c: normal liver areas a: Abundant caveolin-1 reactivities are seen in both the endothelial cells as well as smooth muscle cells in normal areas. P denotes portal tract. C denotes central vein. Bar denotes 154μm. b: Caveolin-1 reactivities are noted on the hepatic artery, capillary venules, and portal vein in the portal tract in normal control liver areas. Arrow heads denote hepatic artery, capillary venules, and portal vein. Bar denotes 32μm. c: Caveolin-1 reactivities are detected in the hepatic sinusoidal lining cells around pericentral zone 3 in normal control liver areas. Arrowheads denotes liver sinusoidal lining cell. Bar denotes 32μm. d-f: lesion. d: Caveolin-1 reactivities are reduced or absent in the hyalinized lesions of sclerosed hemangioma. In residual hemangioma, high expression of caveolin 1 is found in endothelial cells. Caveolin-1 reactivity is almost absent in fibroblasts. Asterisks denote sclerosed portion. Bar denotes 153μm. e: Lesion of liver cavernous hemangioma lesion. Arrowhead denotes endothelial cells. Bar denotes 32μm. f: Lesion of sclerosed hepatic hemangioma. Arrowhead denotes endothelial cell. Bar denotes 32μm. Red rectangles denote the regions seen in 6b or 6c and 6e or 6f, respectively.