Figure 1

The level of H19 transcript in RNA isolated from cells of ascites fluid of a woman patient, determined by RT-PCR or by ISH. (A) 40× magnification of H19 transcripts in the isolated ascites cells determined by ISH analysis. The positive stained cells are marked by black arrows. (B) The H19 transcript in RNA extracted from ascites cells determined by RT-PCR analysis. M: 100 bp ladder, line 1 and 2 are the GADPH and H19 transcripts, respectively. The sizes of the GAPDH internal control and the human H19 PCR products (300 and 270 bp, respectively), are marked (black arrows).