Fig. 2

Phylogenetic relationship of Francisella tularensis ssp. holarctica (Fth) in a wound swab sample from digit IV of the patient’s right hand (A-1825/1) (red, in bold) to Fth isolates from North Rhine-Westphalia (NW), Rhineland-Palatinate (RP), and Hesse (He). Strains belonging to the erythromycin-sensitive major clade B.6 are indicated in blue. For each genome sequence, the year of sampling, the host organism, sampling spot (German federal states), and the known final subclade are given. Host organisms: Be beaver, Br brock, H human, Le Lepus, Sc Sciurus, Wb wild boar. Germany’s federal states: BB Brandenburg, HE Hesse, NI Lower Saxony, NW North Rhine-Westphalia, RP Rhineland-Palatinate. Countries: D Germany, FR France. Asterisk indicates no bacterial isolate, genomic DNA only; for further details, see [13]. The analysis was based on a Mauve alignment for colinear genomes. Genomes were generated by DNA sequencing and mapping of DNA reads to the genome of F. tularensis ssp. holarctica (Fth) strain LVS (for details, see [19]). For the clustering, the neighbor-joining bootstrap method was used, with F. tularensis ssp. holarctica (Fth) strain OSU18 as an outgroup