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Table 1 Antibodies used in this study and the results of staining

From: Spindle cell squamous cell carcinoma exhibiting prominent neutrophil phagocytosis: a case report

Antigen

Type

Clone

Source

Dilution

Retrieval

Results

pan-CK

M

AE1/AE3

Dako

1:100

CB

CK 5/6

M

D5/16 B4

Dako

1:100

CB

CK 17

M

E3

Dako

1:100

CB

EMA

M

E29

Nichirei Bioscience

Prediluted

None

E-cadherin

Rm

24E10

Cell Signaling Technology

1:200

CB

p63

M

4A4

Dako

1:50

CB

+

Vimentin

M

Vim 3B4

Dako

1:500

Trypsin

+

α-SMA

M

1A4

Dako

1:100

CB

+

Desmin

M

D33

Dako

1:100

CB

S-100

Rp

Dako

1:1000

Trypsin

CD31

M

JC70A

Dako

1:50

Trypsin

CD34

M

NU-4A1

Nichirei Bioscience

1:100

CB

CD68

M

PG-M1

Dako

1:50

Trypsin

+

p53

M

DO-7

Dako

1:50

CB

+

Ki-67

M

MIB1

Dako

1:50

CB

+ (approximately 50%)

LAMP-1

M

H5G11

Santa Cruz Biotechnology

1:50

CB

+

Cathepsin B

Rm

D1C7Y

Cell Signaling Technology

1:500

CB

+

Neutrophil elastase

M

NP57

Dako

1:50

None

  1. CK cytokeratin, EMA epithelial membrane antigen, SMA smooth muscle actin, M mouse monoclonal, Rp rabbit polyclonal, Rm rabbit monoclonal, CB autoclave treatment with citrate buffer (pH 6), LAMP-1 lysosomal-associated membrane protein 1.