Fig. 4

Immunohistochemical analysis of the biopsy specimen using immunoperoxidase staining for vimentin (A), α-smooth muscle actin (B), pan-cytokeratin (AE1/AE3) (C), p63 (D), p53 (E), and CD68 (F); hematoxylin was used as the counterstain. Scale bars: 50 μm (A–F). Atypical cells were strongly positive for vimentin (A) and α-smooth muscle actin (B), but not for pan-cytokeratin (C). Nuclear staining of p63 (D) and p53 (E) was observed in a portion of the atypical cells, which also had CD68-positive granules within the cytoplasm (F)