Fig. 3

Immunohistochemical staining of fibroblast growth factor 23 in the resected neurofibromas. Single immunolabeling (peroxidase and diaminobenzidine tetrahydrochloride) of the resected neurofibromas. The upper inset shows hematoxylin and eosin staining. Ossified metaplasia, poorly differentiated foci of cartilage tissue, and osteoclast-like giant cells contained in many mesenchymal tumors are not observed, and dense proliferation of small short spindle-shaped cells against the background of hyaline or myxoma-like stroma are observed. The lower inset shows a negative control using normal rabbit immunoglobulin. The stromal cells in the tissue stained weakly positive using polyclonal rabbit anti-human fibroblast growth factor 23 antibodies