Table 2 Amplification-refractory mutation system primer sequences used for checking c.1924G>C and c.3809A>G variants in healthy populations controls
Primer name | Sequence | Expected size of product fraction | |
|---|---|---|---|
c.1924G>C | EX6F_inn | 5'-CCCAACGCTCATCACTTAG-3' | 173 bp |
EX6R_inn | 5'-GCTTTATTTCCATCTTGTGGAG-3' | 237 bp | |
EX6F_out | 5'-CTATTGGGTAAAGAAGTTGTAAGCAG-3' | 369 bp | |
EX6R_out | 5'-ATTACAAGGGTAAAGGCAGCTAAT-3' | ||
c.3809A>G | EX18F_in | 5'-GTGGGGGATGGGGTCTG-3' | 289 bp |
EX18R_in | 5'-CAAGGCCGGGGAGTCCT-3' | 196 bp | |
EX18Fout | 5'-GTAACTTGAGGTTTCTGCTGCTAT-3' | 451 bp | |
EX18Rout | 5'-AGGTTATAAATCAGTGCCAGGAC-3' |