Table 1 Primer sequences used for polymerase chain reaction amplification of all exons and exon–intron boundaries of ATP7B gene

Exon 1

F5′-CCGGTCCCAAATGAAGG-3′

R5′-TTTTCTCCCACGCCAAG-3′

First part of exon 2

F5′-CAGAGAAGCTGGGATGTTGTAG-3′

R5′-GATAAAGGTCTCTTTGGGTTAGTG-3′

Second part of exon 2

F5′-TTTGAAGCTGCCATCAAGAG-3′

R5′-GACACAAAGAGAAAAGGAGACAAG-3′

Exon 3

F5′-GACAATGAACCCTCACCAAG-3′

R5′-ACTGAGAAGTCTATCCACAAAAGG-3′

Exon 4

F5′-GGGAAGATGTGTTTCTTTGTTC-3′

R5′-CACCGTCTTTAATTCCTGTTTC-3′

Exon 5

F5′-CTGTTGCCATCTGCTTCAC-3′

R5′-CTCATCTTTCTCTTACCCATTCAC-3′

Exon 6

F5′-TCTACTGAGGCACTTTTAGATTCAC-3′

R5′-CTGTTTCAGAGGGTTCACATTAC-3′

Exon 7

F5′-GCAGGTCTTAAACTGTGTCCTC-3′

R5′-GGTGATCCAGTTGTTGCTTC-3′

Exon 8

F5′-GACTGTGCACAAAGCTAGAGG-3′

R5′-CTAAACATGGTGTTCAGAGGAAG-3′

Exon 9

F5′-CAGTGGGAAGACTGATGTTTG-3′

R5′-GTTCTCTGTGAAGTTTCCCTTG-3′

Exon 10

F5′-CATTTCTACCACAGAACTTGTCTTC-3′

R5′-TTGACATCTGAGCCTCTTCC-3′

Exon 11

F5′-GATGGCTTGTTTCATGTTCC-3′

R5′-CTGATTTCCCAGAACTCTTCAC-3′

Exon 12

F5′-GTAATTGCGGGGTCTATAAATG-3′

R5′-TAATAGAAACCTGCAGAAGGAGAG-3′

Exon 13

F5′-CCTCTGACTCTGTCCTGTTTTC-3′

R5′-TTGGTCAAGTTACCTAATCTCCTC-3′

Exon 14

F5′-ATCTGTATTGTGGTCAGTGAGTTG-3′

R5′-TAGGAGAGAAGGACATGGTGAG-3′

Exon 15

F5′-GCTTACAGTTTCCTCTTCCTCTC-3′

R5′-AATTTAGACGCACCCAAGAAC-3′

Exon 16

F5′-CAAATACCTGAGTGCTTCTAATCC-3′

R5′-GGAAGGCTTTTGTTTGTCTTC-3′

Exon 17

F5′-TTCTGCAGGAAAAGACGAAG-3′

R5′-ATCCAGCAAGGGAGAAAGAG-3′

Exon 18

F5′-ATGTGAAGCAGGAGAGTAGGG-3′

R5′-AGCAAATCATTCTGATGGAGAG-3′

Exon 19

F5′-ACTGTGTGCTCCTCTCCATC-3′

R5′-GTCAAAGAGCCATTTCTTTCC-3′

Exon 20

F5′-GAGCTCGCCCTGAAATG-3′

R5′-TGTCCCAGGTGAATGAATG-3′

Exon 21

F5′-CTCAGATGCTGTTGCGTTC-3′

R5′-TCACAGCAGTCATCCTAAATACTC-3′