Fig. 5

Fluorescence in situ hybridization with two split signals (EWSR1 and NR4A3). Both of EWSR1 and NR4A3 split signals showed a pair of split (arrow) and fused (arrow head) patterns of red and green dyes. We counted 50 nuclei of tumor cells and they were assessed as positive if more than 10 % of tumor cells showed split signals. a With EWSR1, the split signal was detected in 44/50 (88 %) of the tumor cells. b With NR4A3, the split signal was detected in 47/50 (94 %) of the tumor cells