Fig. 2From: PRKAR1A-negative familial Cushing’s syndrome: two case reportsAdrenal histology [hematoxylin and eosin stain; original magnifications ×4 (a) and ×10 (b)]. Multiple nodules (2–5 mm in diameter) containing brown pigment can be seen in the adrenal cortex, composed of cells with round to ovoid nuclei with eosinophilic cytoplasm. Many cells with vacuolated, foamy cytoplasm were noted, also in addition to marked vascular congestion. Immunohistochemistry showed positivity for synaptophysin and vimentin, focal positivity for chromogranin A and S100, and a negative result for cytokeratin antibody MNF116Back to article page