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Table 4 Sequence of oligonucleotides for each exon, with the annealing temperatures (T°A) and the length in base pair (bp) of the amplified product

From: Analysis of cystic fibrosis gene mutations in children with cystic fibrosis and in 964 infertile couples within the region of Basilicata, Italy: a research study

Exon

Sequence of primers

T°A

bp

1

GAGAAAGCCGCTAGAGCAAA(CF1F)

55°C

394

TCCTTTACCCCAAACCCAAC(CF1R)

2

TCCAAATCTGTATGGAGACCA(CF2F)

55°C

603

TCAGTGTGAAAATGAGATGTTCC(CF2R)

3

TCTGGCTGAGTGTTTGGTGT(CF3F)

55°C

399

TTTGGAGTTGGATTCATCCTTT(CF3R)

4

AAACTTGTCTCCCACTGTTGC(CF4F)

55°C

453

GGCCTGTGCAAGGAAGTATT(CF4R)

5

GTGCCTAGATGCTGGGAAAT(CF5F)

55°C

393

AAAACTCCGCCTTTCCAGTT(CF5R)

6a

TGCTATGTGCTCCATGTAATGA(CF6AF)

55°C

415

TGCATAGAGCAGTCCTGGTT(CF6AR)

6b

TGCCCATCTGTTGAATAAAAG(CF6BF)

55°C

411

CCCATGAAAGTGAATTTGTGC(CF6BR)

7

TTCCATTCCAAGATCCCTGA(CF7F)

55°C

404

GCACATTTTTGCAAAGTTCA(CF7F)

8

GAATCCTAGTGCTTGGCAAAT(CF8F)

55°C

404

GATCCTCCTTCCAGTTCTACCA(CF8R)

9

GGCCATGTGCTTTTCAAACT(CF9F)

55°C

389

CTCCAAAAATACCTTCCAGCA(CF9R)

10

TGAATCCTGAGCGTGATTTG(CF10F)

55°C

435

TTCATGTGTTTGCAAGCTTCTT(CF10R)

11

GAAGGAAGATGTGCCTTTCAA(CF11F)

55°C

395

CCAAGATACGGGCACAGATT(CF11R)

12

TCAGTGAATCGATGTGGTGAC(CF12F)

55°C

419

ATGAGGCGGTGAGAAAAGGT(CF12R)

13-1

TCATGCTATCAGAATTCACAAGG(CF13F1)

56°C

575

GGGAGTCTTTTGCACAATGG(CF13R1)

13-2

CTGGAGAGTTTGGGGAAAAA(CF13F2)

56°C

449

AAATACCCCCAAGCGATGTA(CF13R2)

14a

CAATGGTGGCATGAACTGT(CF14AF)

55°C

437

GTGGTTCTACTTGTTGATTTTTCAG(CF14AR)

14b

TGGCTTTCTTGTGAGGTTCA(CF4BF)

55°C

446

TGCTTGGGAGAAATGAAACA(CF14BR)

15

GTCGCCAAATAACGATTTCC(CF15F)

55°C

406

AGGTTCAACAAAGGGCACAT(CF15R)

16

TTTGGGTTCTGAATGCGTCT(CF16F)

55°C

388

GGCCAGGTAAGCAGTTCTGA(CF16R)

17a

CTCACCAACATGTTTTCTTTGA(CF17AF)

55°C

399

CCAAAATGAAGTCACATGGTCA(CF17AR)

17b

GAATGGCACCAGTGTGAAAA(CF17BF)

55°C

682

CAATCTGTGTGCATCGGTTT(CF17BR)

18

TGTGCCCTAGGAGAAGTGTG(CF18F)

55°C

335

TGACAGATACACAGTGACCCTCA(CF18R)

19

GCCCGACAAATAACCAAGTG(CF19F)

55°C

399

GCAAGCAGTGTTCAAATCTCA(CF19R)

20

CCAATTCCTTATGCCCAGTT(CF20F)

55°C

408

TGGCTAAGTCCTTTTGCTCA(CF20R)

21

TGATGGTAAGTACATGGGTGTTTC(CF21F)

57°C

578

GGAGCCATACCAGTGAGGAG(CF21R)

22

TCAAATGGTGGCAGGTAGTG(CF22F)

55°C

382

TCACCATGAAGCAGGCATAA(CF22R)

23

CCCATGGTTGAAAAGCTGAT(CF23F)

55°C

417

TGAGTAAAGCTGGATGGCTG(CF23R)

24

GCCTTCTGTCCCAGATCTCA(CF24F)

60°C

362

GAGCAAATGTCCCATGTCAA(CF24R)