Table 1 Intron primers used to amplify the SCN5A and GPD1L exons
From: Brugada syndrome in a family with a high mortality rate: a case report
Gene/Exon | Forward primer (5′ to 3′) (base pairs) | Reverse primer (5′ to 3′) (base pairs) | Product length (base pairs) | Annealing temperature (°C) |
|---|---|---|---|---|
SCN5A | ||||
2 | GAA TCA GGC CCA TTG TCT GTG (21) | GAG TTG CAC AGA AGG GTA GGC (21) | 400 | 59 |
3 | CAC AGT CCA AGG GCT CTG AG (20) | GAA TCA GCG CTA CTC TCA CTC C (22) | 315 | 59 |
4 | ATG CTG CTC AGC TTT CCT TG (20) | GGC AAA AGA GGG TAG AAG CTG (21) | 282 | 57 |
5 | ACG TAA GGA ACC TGG AGA ACC (21) | GCA AGG CAT AGC ACA GCA TAG (21) | 236 | 57 |
61 and 72 | GGT GGT TCT GCT TTG TAA TTC C (22) | CCC AGG CAT ATC CCT CTA GC (20) | 415 | 57 |
8 | CCG TGC TTG TTC TTG CCT TC (20) | GTC TGT CCT CTG TCT GGG TCT C (22) | 399 | 59 |
9 | CCC TCA CCA GCA TGA TGT TTC (21) | CCT GGC AGG TAA GGG AGA CAA (21) | 231 | 59 |
10 | GAC GGC ATG GAA CAA AGT CTC (21) | CCC ACA CTT GCT GTC CCT TG (20) | 213 | 59 |
11 | CCA GTG AGG GTG ACC TCT GC (20) | CAG TCA GGT GAG GGC TTA GAG G (22) | 281 | 61 |
12 | GCT GCA CAA AGT CTC AAT GAT G (22) | CCA CCC TGG AAA AGC TAG AAC (21) | 267 | 57 |
13 | GAA GAG CAC ATG TCA TGG TCG TG (23) | CAG ACC TGC TGT GGT GCC TG (20) | 456 | 61 |
14 | GCT GAC GCA AAT CTC CTG AT (20) | GCT TAA ATG ACC TGG GGT TG (20) | 187 | 57 |
15 | CTA ACT CAT TGG CTG TCC CC (20) | CTT ACC CAT GAA GGC TGT GC (20) | 347 | 57 |
16 | GCC AGG GAG TCT TTC CAT CC (20) | GTG ATG ACC TCA GAT TGG GTT G (22) | 296 | 59 |
17 | CTT CAC AAG GTC CCC TCC TC (20) | TGG GTA GAT GGA TTG ATA GAA GG (23) | 488 | 57 |
18 | GGA CTG GAT GGC TTG GCA TG (20) | GCT CTG GGC CCT GTA TAT GTA GG (23) | 553 | 59 |
19 | GCA TGG GCA GGG TCT GAA AC (20) | GCT TCA GGG ACA AAG GCA TG (20) | 280 | 59 |
20 | GCT ACT CAG CCC ACA CTC ACA C (22) | CTC TGG GTG GAA CTG AGG CTA G (22) | 224 | 61 |
21 | CAT TAG ATG TGG GCA TTC ACA G (22) | CCA GCT GGA GAC CTC CTT TC (20) | 294 | 57 |
22 | GCT TCA TGT CCA CCT TGT CTG (21) | CAA TGG GTT TCT CCT TCC TG (20) | 252 | 57 |
23 | GCC TAC TGT CTG TCC CCA AC (20) | GGC CAT AGG ACA TCA GAA GC (20) | 295 | 59 |
24 | GGT CTT GAA AAG GGC ATG TG (20) | CCA TTG GGA GGA AGG AAG TC (20) | 386 | 57 |
25 | GCT TCT GGC TTC ATC TGT CC (20) | CAG ACA CTG ATT CCC TGG TG (20) | 228 | 57 |
26 | CCA GCC TGT CTG ATC TCC CTG (21) | CCA ACA GGG AAG GTG AGA TGG (21) | 283 | 61 |
27 | CAG AAT GAG GTT GGT GCC TTC (21) | CTG GGC TGA AAG ACT GTG AAG (21) | 246 | 57 |
28 | CCT GCT GAG CAC TTT CCA TTT G (22) | GGT TGT ACA TGG CAT TCA GCA G (22) | 396 | 57 |
29.13 | GCC ACC AGT AGC CAC AGT CTC (21) | GGC CTC TGG GTC AAA TTT CTC (21) | 638 | 57 |
29.2 | GTG CCT CTT CCA GAT CAC CAC (21) | GAT AAC CAT GGC CGA CAC CTC (21) | 619 | 57 |
29.3 | CCT GAG TGA GGA CGA CTT CGA (21) | GGC TGC TTT TCA GTG TGT CCT (21) | 736 | 57 |
GPD1L | ||||
1 | ACG GTC CAG GCG GCT ACA TT (20) | GCT TCT GTG CGC TCA GTA ACG (21) | 364 | 57 |
2 | CTC TCC CGC CCA AGT GAG TT (20) | CCA AGT GCT GCT CAG CCA AG (20) | 297 | 57 |
3 | CCT TGT GGA CAG GCA AGG TT (20) | CAA TGT CTA GCC CTG GGC AG (20) | 240 | 57 |
4 | TCC CAT GCC TCT TTG ATT TG (20) | ACA TCA AAT GTC CAG ACT CCT GG (23) | 243 | 57 |
5 | GGC TGT TAT TAA TAT CCT TGT TGT C (25) | CAC AGA GCC CAT TCT TGA GGA (21) | 236 | 57 |
6 | GCA TCT GGG CTT TGT CAT CT (20) | CCA AGC CAG GAG TCT CAA GT (20) | 322 | 53 |
7 | CAG CAT AGC AAG ACC CAT TCT (21) | GGC TGG AAG TCC TGA GAA TAG (21) | 299 | 53 |
8 | CTG AGG TAA ATC CAG TGG CC (20) | CAT CAT GTT GCC AAG TCC TG (20) | 238 | 53 |