Skip to main content

Table 1 Intron primers used to amplify the SCN5A and GPD1L exons

From: Brugada syndrome in a family with a high mortality rate: a case report

Gene/Exon Forward primer (5′ to 3′) (base pairs) Reverse primer (5′ to 3′) (base pairs) Product length (base pairs) Annealing temperature (°C)
SCN5A     
2 GAA TCA GGC CCA TTG TCT GTG (21) GAG TTG CAC AGA AGG GTA GGC (21) 400 59
3 CAC AGT CCA AGG GCT CTG AG (20) GAA TCA GCG CTA CTC TCA CTC C (22) 315 59
4 ATG CTG CTC AGC TTT CCT TG (20) GGC AAA AGA GGG TAG AAG CTG (21) 282 57
5 ACG TAA GGA ACC TGG AGA ACC (21) GCA AGG CAT AGC ACA GCA TAG (21) 236 57
61 and 72 GGT GGT TCT GCT TTG TAA TTC C (22) CCC AGG CAT ATC CCT CTA GC (20) 415 57
8 CCG TGC TTG TTC TTG CCT TC (20) GTC TGT CCT CTG TCT GGG TCT C (22) 399 59
9 CCC TCA CCA GCA TGA TGT TTC (21) CCT GGC AGG TAA GGG AGA CAA (21) 231 59
10 GAC GGC ATG GAA CAA AGT CTC (21) CCC ACA CTT GCT GTC CCT TG (20) 213 59
11 CCA GTG AGG GTG ACC TCT GC (20) CAG TCA GGT GAG GGC TTA GAG G (22) 281 61
12 GCT GCA CAA AGT CTC AAT GAT G (22) CCA CCC TGG AAA AGC TAG AAC (21) 267 57
13 GAA GAG CAC ATG TCA TGG TCG TG (23) CAG ACC TGC TGT GGT GCC TG (20) 456 61
14 GCT GAC GCA AAT CTC CTG AT (20) GCT TAA ATG ACC TGG GGT TG (20) 187 57
15 CTA ACT CAT TGG CTG TCC CC (20) CTT ACC CAT GAA GGC TGT GC (20) 347 57
16 GCC AGG GAG TCT TTC CAT CC (20) GTG ATG ACC TCA GAT TGG GTT G (22) 296 59
17 CTT CAC AAG GTC CCC TCC TC (20) TGG GTA GAT GGA TTG ATA GAA GG (23) 488 57
18 GGA CTG GAT GGC TTG GCA TG (20) GCT CTG GGC CCT GTA TAT GTA GG (23) 553 59
19 GCA TGG GCA GGG TCT GAA AC (20) GCT TCA GGG ACA AAG GCA TG (20) 280 59
20 GCT ACT CAG CCC ACA CTC ACA C (22) CTC TGG GTG GAA CTG AGG CTA G (22) 224 61
21 CAT TAG ATG TGG GCA TTC ACA G (22) CCA GCT GGA GAC CTC CTT TC (20) 294 57
22 GCT TCA TGT CCA CCT TGT CTG (21) CAA TGG GTT TCT CCT TCC TG (20) 252 57
23 GCC TAC TGT CTG TCC CCA AC (20) GGC CAT AGG ACA TCA GAA GC (20) 295 59
24 GGT CTT GAA AAG GGC ATG TG (20) CCA TTG GGA GGA AGG AAG TC (20) 386 57
25 GCT TCT GGC TTC ATC TGT CC (20) CAG ACA CTG ATT CCC TGG TG (20) 228 57
26 CCA GCC TGT CTG ATC TCC CTG (21) CCA ACA GGG AAG GTG AGA TGG (21) 283 61
27 CAG AAT GAG GTT GGT GCC TTC (21) CTG GGC TGA AAG ACT GTG AAG (21) 246 57
28 CCT GCT GAG CAC TTT CCA TTT G (22) GGT TGT ACA TGG CAT TCA GCA G (22) 396 57
29.13 GCC ACC AGT AGC CAC AGT CTC (21) GGC CTC TGG GTC AAA TTT CTC (21) 638 57
29.2 GTG CCT CTT CCA GAT CAC CAC (21) GAT AAC CAT GGC CGA CAC CTC (21) 619 57
29.3 CCT GAG TGA GGA CGA CTT CGA (21) GGC TGC TTT TCA GTG TGT CCT (21) 736 57
GPD1L     
1 ACG GTC CAG GCG GCT ACA TT (20) GCT TCT GTG CGC TCA GTA ACG (21) 364 57
2 CTC TCC CGC CCA AGT GAG TT (20) CCA AGT GCT GCT CAG CCA AG (20) 297 57
3 CCT TGT GGA CAG GCA AGG TT (20) CAA TGT CTA GCC CTG GGC AG (20) 240 57
4 TCC CAT GCC TCT TTG ATT TG (20) ACA TCA AAT GTC CAG ACT CCT GG (23) 243 57
5 GGC TGT TAT TAA TAT CCT TGT TGT C (25) CAC AGA GCC CAT TCT TGA GGA (21) 236 57
6 GCA TCT GGG CTT TGT CAT CT (20) CCA AGC CAG GAG TCT CAA GT (20) 322 53
7 CAG CAT AGC AAG ACC CAT TCT (21) GGC TGG AAG TCC TGA GAA TAG (21) 299 53
8 CTG AGG TAA ATC CAG TGG CC (20) CAT CAT GTT GCC AAG TCC TG (20) 238 53
  1. 1Exon 6 of SCN5A is only present in transcript variant 3, which is represented by the reference sequence NG_008934.1. 2Alternate designation 6, numbering commonly used in the literature. The same reasoning applies to the remaining exons (8 to 29). 3Several pairs of primers were synthesized to polymerase chain reaction-amplify exon SCN5A 29 owing to its large sizes.