Figure 5

Intracellular localization of high mobility group box chromosomal protein 1 in the hepatic tissues. Cells were double-stained with anti-high mobility group box chromosomal protein 1 antibodies and propidium iodide and analyzed by confocal microscopy. In the control cells, high mobility group box chromosomal protein 1 was located in the cytoplasm. In the hepatocellular carcinoma group, high mobility group box chromosomal protein 1 was mainly located in the nucleus. In the actinomycosis-affected cells, a high level of high mobility group box chromosomal protein 1 was found in both the cytoplasm and nucleus (original magnification, ×400). HMGB1, high mobility group box chromosomal protein 1; HCC, hepatocellular carcinoma; PI, propidium iodide.