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Table 1 Summary of assays performed

From: Rotational thromboelastometry and multiple electrode platelet aggregometry in four patients with abnormal routine coagulation studies before removal of epidural catheters after major surgery: a case series and research study

Test Vial Apparatus Place of analysis Normal range
ROTEM® using the following reagents: EXTEM, FIBTEM®, INTEM, APTEM, NATEM, HEPTEM®, recording the following results: CT (Clotting time), CFT (Clot formation time), Alpha-Angle (AA), MCF (Maximum Clot Firmness), ML (Maximum Lysis). 2.7mL citrate tubes for ROTEM® analysis (3.2% citrate, BD Vacutainer® Systems, Plymouth, UK) ROTEM®, Pentapharm, Munich, Germany Point-of-care laboratory, Intensive Care Unit See Table 2.
Multiplate® using the following agonists: adenosine diphosphate, collagen, thrombin receptor activator, recording the following results for each agonist: area under curve (AUC), aggregation (AU), velocity (AU/min). 3.0mL Hirudin tubes. Dynabyte GmbH, Munich, Germany. Multiplate®, Roche, Basel, Switzerland See Table 2.
aPTT: activated partial thromboplastin time 2.7mL citrate tubes (3.2% citrate, BD Vacutainer® Systems, Plymouth, UK) PTT-Automate, Stago (Asnière sur Seine, France) Hospital’s usual clinical chemistry laboratory 28 to 45 seconds at the time of the study
PT-INR: prothrombin time-international normalized ratio Stago prothrombin complex assay, Stago calibrated by Equalis, (Uppsala, Sweden) ≤1.2
Plc: platelet count 3.0mL K2EDTA tubes (BD Vacutainer® Systems, Plymouth UK) Sysmex XE 5000 cell counter, Sysmex Corp., (Kobe, Japan).   165 to 387 million/mL and 145 to 387 million/mL for women and men respectively