Figure 2
Molecular findings. (a) Agarose gel electrophoresis to detect the rearrangement of the immunoglobulin heavy chain (IgH) rearrangement PCR products (semi-nested PCR). Lane 1: patient sample; lane 2: negative control (no DNA); lane 3: polyclonal control; lane 4: monoclonal control; lane MK: DNA ladder (501, 404, 331, 242, 190,147,111, 67, 34 bp fragments). (b) Agarose gel electrophoresis to detect BCL2/JH PCR products (nested PCR). Lane 1: negative control (no DNA); lane 2: patient sample; lane 3: BCL2 MBR positive control (plasmidic DNA containing BCL2/MBR rearrangement); lane MK: DNA ladder (501, 404, 331, 242, 190,147,111, 67, 34 bp fragments).