Detection of aberrant DNA methylation in the giant fibroadenoma. A: MS-MLPA analysis of DNA isolated from non-giant fibroadenoma. None of the analyzed regions are methylated. Only the PCR products from control probes are detected. B and C: MS-MLPA analysis of DNA isolated from the giant fibroadenoma. The methylation specific peaks are marked with an asterisk (*). Panel B shows the presence of methylation in BRCA2, CD44 and ESR1 genes and panel C shows the methylation of WT1, ESR1 and MGMT genes.