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Figure 2 | Journal of Medical Case Reports

Figure 2

From: Treatment of ovarian cancer ascites by intra-peritoneal injection of diphtheria toxin A chain-H19 vector: a case report

Figure 2

Pharmacokinetics of DTA-H19 in blood and urine of the patient after the first treatment. Blood and urine samples were collected before and after two, six, 12, 24, 48, 72 hours and one week from each of the treatments one to three. The urine samples were transferred into test tubes and frozen at -20°C until analysis. Blood samples were kept for 10 min at room temperature and then centrifuged for 10 min at 3500 rpm. The serum was transferred into a new test tube and kept at -20°C until analysis. Total DNA was isolated from blood and urine. The DNA samples were submitted to quantitative real time PCR analysis using specific probe. Panels A and B show the result for blood and urine samples, respectively.

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