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Table 1 List of primers covering the entire exons and intron-exon boundaries for the ADA gene

From: A novel mutation in the ADA gene causing severe combined immunodeficiency in an Arab patient: a case report

Exon Primer sequence Tm (°C) Size (bp)
1 F-5′-TGTGTGTTTCTGCGACGAGC-3′ 55 692
  R-5′-TGTCCCTGATTAGCCCGCAA-3′   
2 F-5′-GCAGCCAGCCAGTAAAATG-3′ 55 366
  R-5′-TGTCCTCACAGTCCCACTTC-3′   
3 F-5′-GTCCACCACTCACTGTTTTG-3′ 55 384
  R-5′-AGTCCATCACACCCACATC-3   
4 F-5′-TGTTCCCAACCCCTTTCTTCC-3′ 55 556
  R-5′-AAATGGGCCAGACTCACTTCAG-3′   
5 F-5′-CCCAAAGCCTCCTCTTCCTCCT-3' 55 377
  F-5′-AGGTCTCCAGTTGTTTCATG-3′   
6 F-5′-TAGGCTGGGAGGTCTCTC-3′ 55 315
  R-5′-ACCCAACAAAGACACACTC-3′   
7-9 F-5′-ATGCTGTTGAAGCAGGCAGCATGACTAGGA-3' 60 739
  F-5′-TGCCTGCTTCCCAGGGTGTCGAAGAGATTT-3′   
10 F-5′-AGGATCAAAGGCGGGTGAAC-3′ 55 312
  R-5′-TCCCTCTCTCCAAAGATTCCAG-3′   
11 F-5′-AGGATCAAAGGCGGGTGAAC-3′ 55 238
  R-5′-TCCCTCTCTCCAAAGATTCCAG-3′   
12 F-5′-TCTGAAGCCCAGTCCCAAAG-3′ 55 363
  R-5′-AAATGTTGCTCAGCCCCAC-3′   
  1. Corresponding Tm and size in bp are indicated. PCR conditions as described in the case presentation.