Table 1 List of primers covering the entire exons and intron-exon boundaries for the ADA gene
Exon | Primer sequence | Tm (°C) | Size (bp) |
|---|---|---|---|
1 | F-5′-TGTGTGTTTCTGCGACGAGC-3′ | 55 | 692 |
|  | R-5′-TGTCCCTGATTAGCCCGCAA-3′ |  |  |
2 | F-5′-GCAGCCAGCCAGTAAAATG-3′ | 55 | 366 |
|  | R-5′-TGTCCTCACAGTCCCACTTC-3′ |  |  |
3 | F-5′-GTCCACCACTCACTGTTTTG-3′ | 55 | 384 |
|  | R-5′-AGTCCATCACACCCACATC-3 |  |  |
4 | F-5′-TGTTCCCAACCCCTTTCTTCC-3′ | 55 | 556 |
|  | R-5′-AAATGGGCCAGACTCACTTCAG-3′ |  |  |
5 | F-5′-CCCAAAGCCTCCTCTTCCTCCT-3' | 55 | 377 |
|  | F-5′-AGGTCTCCAGTTGTTTCATG-3′ |  |  |
6 | F-5′-TAGGCTGGGAGGTCTCTC-3′ | 55 | 315 |
|  | R-5′-ACCCAACAAAGACACACTC-3′ |  |  |
7-9 | F-5′-ATGCTGTTGAAGCAGGCAGCATGACTAGGA-3' | 60 | 739 |
|  | F-5′-TGCCTGCTTCCCAGGGTGTCGAAGAGATTT-3′ |  |  |
10 | F-5′-AGGATCAAAGGCGGGTGAAC-3′ | 55 | 312 |
|  | R-5′-TCCCTCTCTCCAAAGATTCCAG-3′ |  |  |
11 | F-5′-AGGATCAAAGGCGGGTGAAC-3′ | 55 | 238 |
|  | R-5′-TCCCTCTCTCCAAAGATTCCAG-3′ |  |  |
12 | F-5′-TCTGAAGCCCAGTCCCAAAG-3′ | 55 | 363 |
|  | R-5′-AAATGTTGCTCAGCCCCAC-3′ |  |  |