(a) Number of tumor cells obtained from ascites. From 16 February 2007 (day 1), we started to drain 3000 ml of ascites and counted the number of tumor cells in the hypogastric area. After the second drainage of ascites, we infused DC+AK cells intraperitoneally with IL2. Three days later, 2200 ml of ascites was drained and the number of tumor cells was counted. A drastic decrease in the tumor cell count (1/77) was observed: from 4.6 × 108 cells to 6 × 106 cells. From 23 March (day 36), we initiated drainage from the epigastric area. The number of tumor cells decreased from 6.6 × 108 (day 36) to 0 (day 71) after the fifth immunotherapy with DC+AK cells and TIL. (b) Cytotoxic activity of autologous and allogeneic effector cells. Cytotoxic activity of TIL was examined by the 51Cr-releasing test. Tumor cells obtained from ascites were labeled with Na2
51CrO4, incubated with autologous effector (TIL) cells and allogeneic effector (MT-116) cells. 51Cr release was counted with a gamma counter. TIL showed a high cytotoxic activity against autologous tumor cells (black filled diamond), but MT-116 did not (black filled square).