Testicular seminomatous mixed germ cell tumor with choriocarcinoma and teratoma with secondary somatic malignancy: a case report
© Aneja et al.; licensee BioMed Central Ltd. 2014
Received: 30 April 2013
Accepted: 7 October 2013
Published: 1 January 2014
Testicular tumors are a heterogeneous group of neoplasms exhibiting diverse histopathology and can be classified as seminomatous and non-seminomatous germ cell tumor types. Mixed germ cell tumors contain more than one germ cell component and various combinations have been reported. Here, we present a rare case of a mixed germ cell tumor composed of seminoma, choriocarcinoma and teratoma with a secondary somatic malignancy.
A 31-year-old Caucasian man presented with splenic rupture to our hospital. A right-sided testicular swelling had been present for 6 months and his alpha-fetoprotein, beta-human chorionic gonadotropin, and lactose dehydrogenase were increased. An ultrasound of his scrotum revealed an enlarged right testis with heterogeneous echogenicity. Multiple hypervascular lesions were noted in his liver and spleen. He underwent transcatheter embolization therapy of his splenic artery followed by splenectomy and right-sided orchiectomy. A computed tomography scan also showed metastasis to both lungs. During his last follow up after four cycles of cisplatin-based chemotherapy, the level of tumor markers had decreased, decreases in the size of his liver and pulmonary lesions were noted but new sclerotic lesions were evident in his thoracolumbar region raising concern for bony metastasis.
Prognosis of testicular tumor depends mainly on the clinical stage, but emergence of a sarcomatous component presents a challenge in the treatment of germ cell tumors and the histological subtype of this component can be used as a guide to specific chemotherapy in these patients.
KeywordsMixed germ cell tumors Rhabdomyosarcoma Sarcomatous component
Testicular tumors are a heterogeneous group of neoplasms exhibiting diverse histopathology, variable clinical course and prognosis. Of these tumors, 30 to 50% are classified as mixed germ cell tumors (GCT) and several studies have assessed the frequency of various histological elements seen in these tumors[3, 4]. The combination of seminoma and choriocarcinoma is reported to be extremely rare. Further, the presence of a sarcomatous component (SC) in a GCT is an infrequent phenomenon with great implications on clinical outcome and prognosis. We report a rare case of mixed GCT with combination of seminoma, choriocarcinoma and teratoma with a secondary somatic malignancy of rhabdomyosarcoma.
A 31-year-old Caucasian man was referred to our hospital for splenic rupture with active arterial extravasation. During clinical evaluation, he mentioned right testicular swelling; the swelling had been enlarging for 6 months. Transcatheter embolization therapy of the inferior pole of his splenic artery was performed for what was clinically thought to be a hypervascular metastasis in the spleen. Multiple hypervascular lesions were also noted in his liver. An ultrasound of his scrotum revealed an enlarged right testis with heterogeneous echogenicity measuring 5×4×3cm. Serum tumor markers revealed a beta-human chorionic gonadotropin (hCG) of 3804mIU/mL (<5mIU/mL), lactose dehydrogenase (LDH) of 196U/L (100 to 190U/L) and an alpha-fetoprotein (AFP) of 47.7ng/mL (0.0 to 9.0ng/mL). He underwent right-sided orchiectomy and splenectomy. No lymph node dissection was performed.
On gross pathological examination, a 5.1×5×2.5cm heterogeneous testicular mass, exhibiting tan-white to yellow to hemorrhagic areas was evident. No gross involvement of his tunica albuginea or spermatic cord was noted.
He was placed on cisplatin-based chemotherapy and he has received four rounds to date. Bleomycin was omitted from the first round only because he was unable to perform pulmonary function tests; his exhalation time was too short and the end test criteria were not met. A repeat pulmonary function test 2 weeks later revealed normal air flow and normal diffusion capacity. By the end of the fourth cycle, his AFP had returned to normal and his beta-hCG had decreased to 137mIU/mL and LDH was 196U/L.
During his last follow up, we observed that his pulmonary and liver lesions had decreased in size but new sclerotic lesions had developed in his thoracolumbar spine which raised concerns of bony metastatic involvement. A biopsy of his L5 vertebral body however, showed no evidence of metastatic disease.
Several studies have reported specific combinations of different GCT elements in mixed GCT of testis[2, 3]. Mostofi in classifying more than 6000 testicular tumors found >1 histological patterns in approximately 60% of cases with the most frequent combination of embryonal carcinoma, yolk sac tumor and choriocarcinoma. Statistical analysis of possible combinations by Mosharafa et al. revealed 10 possible pair combinations with the strongest correlation between teratoma and yolk sac tumor. To the best of our knowledge, the combination of seminoma, choriocarcinoma and teratoma with rhabdomyosarcoma as a secondary somatic malignancy has not been reported so far.
According to the World Health Organization, a teratoma with somatic malignancy can be defined as a teratoma containing a malignant component of a type typically encountered in other organs and tissues, for example sarcomas and carcinomas. Absence of this combination of germ cell elements in separate studies by Malagón et al. and Guo et al. of 46 and 33 cases respectively of GCT with a SC further support this as a rare combination of germ cell elements exhibiting a SC[5, 8].
There are several hypotheses regarding the origin of SC in GCT. As this component tends to occur in GCTs that contain a teratomatous component, it has been suggested that SC may arise from malignant transformation of teratomatous mesenchyme. However, reports of SC in GCT without teratomatous component point towards other mechanisms such as aberrant differentiation of primitive germ cells and de-differentiation of blastomatous stroma in yolk sac component[5, 9].
The majority of the sarcomatous tumor of the testis is an element of germ cell neoplasia but, occasionally, can be seen as an invasion from a paratesticular sarcoma or as a primary intratesticular sarcoma. However, this is a rare occurrence. Stewart et al. found only six cases, of which four were paratesticular and two were testicular, in a 30-year review of intrascrotal rhabdomyosarcomas. Erbay et al. while reporting a case of primary testicular rhabdomyosarcoma emphasized investigating for the teratomatous elements in the primary tumor due to the rarity of this entity. They also advised serial serologic evaluation of beta-hCG and AFP to exclude undetected GCT metastases. However, it is also important to differentiate SC from focal atypical stromal proliferations commonly seen in teratoma. These areas usually lack marked cytological atypia and do not form an expansile nodule whereas the sarcomatous cells show prominent cytologic atypia, brisk mitotic activity and complex growth pattern. The histological analysis in our case clearly showed latter areas present within the teratomatous component.
Development of SC presents a challenge in the treatment of testicular GCT. Malagón et al. reported a statistically significant difference in survival (P<0.001) for patients having a SC in their GCT when compared to age and stage-matched patients with GCTs without SC. Many studies have revealed resistance to the cisplatin-based chemotherapy in GCTs with SC[12–15]. Donadio et al. emphasized in their study the importance of histological type of SC as a guide to specific chemotherapy in their patients, for example GCT with rhabdomyosarcoma responds well to a doxorubicin-based regimen whereas GCTs with secondary adenocarcinoma benefit from 5-fluorouracil-based chemotherapy. Guo et al. suggested in their study that the SC confined to the primary testicular GCT may not have as high a risk of mortality as those at a comparable stage without a SC. They further indicated that patients with SC in metastasis may benefit from sarcomatous-oriented drugs. Alternatively, SC may occur in primary testicular GCT or metastasis.
We report the first known example of a GCT with an unusual combination of seminoma, choriocarcinoma and teratoma with a somatic malignancy. This case not only reflects the heterogeneity in the biology of GCT of the testis but also highlights the aggressiveness of these tumors and challenges in treatment due to the emergence of a SC in them.
Written informed consent was obtained from the patient for publication of this manuscript and accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
Germ cell tumor
Human chorionic gonadotropin
We acknowledge the cooperation of our patient, as well as the patient’s family. This paper was not funded by any external source.
- Bahrami A, Ro JY, Ayala AG: An overview of testicular germ cell tumors. Arch Pathol Lab Med. 2007, 131: 1267-1280.PubMedGoogle Scholar
- Cheville JC: Classification and pathology of testicular germ cell and sex cord-stromal tumors. Urol Clin North Am. 1999, 26: 595-609. 10.1016/S0094-0143(05)70201-9.View ArticlePubMedGoogle Scholar
- Mostofi FK: Proceedings: Testicular tumors. Epidemiologic, etiologic, and pathologic features. Cancer. 1973, 32: 1186-1201. 10.1002/1097-0142(197311)32:5<1186::AID-CNCR2820320527>3.0.CO;2-8.View ArticlePubMedGoogle Scholar
- von Hochstetter AR, Hedinger CE: The differential diagnosis of testicular germ cell tumors in theory and in practice. A critical analysis of two major systems of classification and review of 389 cases. Virchows Arc A Pathol Anat Histol. 1982, 396: 247-10.1007/BF00431386.View ArticleGoogle Scholar
- Malagón HD, Valdez AM, Moran CA, Suster S: Germ cell tumors with sarcomatous components: a clinicopathologic and immunohistochemical study of 46 cases. Am J Surg Pathol. 2007, 31: 1356-1362. 10.1097/PAS.0b013e318033c7c4.View ArticlePubMedGoogle Scholar
- Mosharafa AA, Foster RS, Leibovich BC, Ulbright TM, Bihrle R, Einhorn LH, Donohue JP: Histology in mixed germ cell tumors. Is there a favorite pairing?. J Uro. 2004, 171: 1471-1473. 10.1097/01.ju.0000116841.30826.85.View ArticleGoogle Scholar
- Woodword PJ, Heidenreich A, Looijenga LHJ, Oosterhuis JW, Mcleod DG, Moller H, Manivel JC, Mostofi FK, Hailemariam S, Parkinson MC, Grigor K, True L, Jacobsen GK, Oliver TD, Talerman A, Kaplan GW, Ulbright TM, Sesterhenn IA, Rushton HG, Michael H, Reuter VE: Germ cell tumors. World Health Organization Classification of Tumors: Pathology and Genetics of Tumors of the Urinary System and Male Genital Organs. Edited by: Eble J, Sauter G, Epstein J, Sesterhenn I. 2004, Lyon: IARC Press, 221-249.Google Scholar
- Guo CC, Punar M, Contreras AL, Tu SM, Pisters L, Tamboli P, Czerniak B: Testicular germ cell tumors with sarcomatous components: an analysis of 33 cases. Am J Surg Pathol. 2009, 33: 1173-1178. 10.1097/PAS.0b013e3181adb9d7.View ArticlePubMedGoogle Scholar
- True LD, Otis CN, Delprado W, Scully RE, Rosai J: Spermatocytic seminoma of testis with sarcomatous transformation. A report of five cases. Am J Surg Pathol. 1988, 12: 75-82. 10.1097/00000478-198802000-00001.View ArticlePubMedGoogle Scholar
- Stewart LH, Lioe TF, Johnston SR: Thirty year review of intrascrotal rhabdomyosarcoma. Brit J Urol. 1991, 68: 418-420. 10.1111/j.1464-410X.1991.tb15364.x.View ArticlePubMedGoogle Scholar
- Erbay ME, Tarhan F, Barısık NO, Kuyumcuoglu U: A case of testicular rhabdomyosarcoma. Int Urol Nephrol. 2004, 36: 73-75.View ArticlePubMedGoogle Scholar
- Michael H, Lucia J, Foster RS, Ulbright TM: The pathology of late recurrence of testicular germ cell tumors. Am J Surg Pathol. 2000, 24: 257-273. 10.1097/00000478-200002000-00012.View ArticlePubMedGoogle Scholar
- Motzer RJ, Amsterdam A, Prieto V, Sheinfeld J, Murty VV, Mazumdar M, Bosl GJ, Chaqanti RS, Reuter VE: Teratoma with malignant transformation: diverse malignant histologies arising in men with germ cell tumors. J Urol. 1998, 159: 133-138. 10.1016/S0022-5347(01)64035-7.View ArticlePubMedGoogle Scholar
- Toner GC, Panicek DM, Heelan RT, Geller NL, Lin SY, Bajorin D, Motzer RJ, Scher HI, Herr HW, Morse MJ: Adjunctive surgery after chemotherapy for nonseminomatous germ cell tumors: recommendations for patient selection. J Clin Oncol. 1990, 8: 1683-1694.PubMedGoogle Scholar
- Ulbright TM, Loehrer PJ, Roth LM, Einhorn LH, Williams HD, Clark SA: The development of non-germ cell malignancies within germ cell tumors. A clinicopathologic study of 11 cases. Cancer. 1984, 54: 1824-1833. 10.1002/1097-0142(19841101)54:9<1824::AID-CNCR2820540910>3.0.CO;2-J.View ArticlePubMedGoogle Scholar
- Donadio AC, Motzer RJ, Bajorin DF, Kantoff PW, Sheinfeld J, Houldsworth J, Chaqanti RS, Bosl GJ: Chemotherapy for teratoma with malignant transformation. J Clin Oncol. 2003, 21: 4285-4291. 10.1200/JCO.2003.01.019.View ArticlePubMedGoogle Scholar
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