Plasma exchange in severe leptospirosis with multi-organ failure: a case report
© Taylor and Karamadoukis; licensee BioMed Central Ltd. 2013
Received: 9 January 2013
Accepted: 7 May 2013
Published: 28 June 2013
Leptospirosis presents with disease of variable severity; multi-organ failure can occur. In this situation, plasma exchange has been used with positive results, although the mechanism of action has not been fully explained.
A 67-year-old Caucasian man developed severe leptospirosis with marked icterus, acute kidney injury and cardiorespiratory failure, after exposure to livestock. On the basis of previous reported cases, he was treated with plasma exchange. This led to a rapid improvement in his bilirubin level, cardiac and respiratory function, followed by renal function.
We discuss the pathophysiology of the disease, and suggest that plasma exchange has a role in the treatment of severe sepsis caused by leptospirosis as well as immune complex-mediated organ injury.
Leptospirosis is caused by the Leptospira species of Spirochaetes and is presumed to be the world’s most common zoonosis. Infection usually follows contact with the urine of infected animals through broken or water-soaked skin or the conjunctivae. The largest reservoir of infection is rats, although leptospires have been isolated from cattle populations, and contact with livestock is an established risk factor . In the United Kingdom there are around 60 serologically proven cases per year , although the incidence is far higher in tropical countries . These are likely to be underestimates as many have mild or subclinical infection, and serological testing is imperfect and not always performed.
The disease follows a biphasic course . An initial ‘infective’ or ‘septicemic’ phase lasts for 4 to 7 days during which leptospires can be found in the blood and the cerebrospinal fluid. Symptoms at this stage are non-specific and include fever, headache, myalgia, abdominal pain and uveitis. This is followed by a period of 1 to 3 days during which the fever resolves. An ‘immune’ phase follows, during which leptospires are excreted in the urine and anti-Leptospira immunoglobulin M (IgM) antibodies develop in the blood . At this stage patients redevelop the initial symptoms, including fever and sometimes aseptic meningitis. Five to 10 percent of patients progress to the severe form of the disease which is also known as Weil’s disease . This comprises jaundice due to hepatocellular dysfunction rather than hepatic necrosis and multi-organ involvement including acute kidney injury (AKI), pulmonary hemorrhage and adult respiratory distress syndrome.
Penicillins may shorten the duration of disease if given during the infective phase and have been shown to reduce urinary shedding of leptospires . Treatment of severe disease after this stage is supportive, and may require intensive care support. There is also evidence of benefit from methylprednisolone in severe leptospirosis if given before the onset of multi-organ failure . There is a considerable risk of death especially in cases with pulmonary involvement, where reported survival rates are as low as 16% . However, there have been reports of the use of plasma exchange in such patients with positive outcomes [6–8]. We report successful treatment with plasma exchange in severe leptospirosis and discuss the possible mechanisms of action.
A 67-year-old, male Caucasian livestock hauler presented to our acute medical unit with a 5-day history of lethargy and myalgia. He complained of dysuria with dark, malodorous urine, vomiting and hiccoughs. He was previously well with a history of bilateral inguinal hernia repair only. He took no regular medications but had been prescribed trimethoprim for presumed urinary tract infection 1 day previously.
On examination he was icteric, his temperature was 37.6°C and he was clinically dehydrated. Initial blood pressure (BP) was 118/65mmHg, heart rate 100 beats per minute. He had right upper quadrant abdominal tenderness but no evidence of peritonitis.
Initial blood tests revealed hemoglobin 78g/L (7.8g/dL), total white cell count 20×103/μL, neutrophils, 18.3×103/μL and platelet count 44×103/μL. Coagulation parameters were in the normal range. Serum creatinine was 4.87mg/dL (431umol/L) and serum urea 63.9mg/dL (22.8mmol/L). Bilirubin was 12.9mg/dL (221μmol/L), alkaline phosphatase 236IU/L, and alanine transaminase (ALT) 152IU/L. C-reactive protein level was 280.2mg/L (2660mmol/L). Urine dipstick was positive for blood, protein and bilirubin and Proteus mirabilis was isolated on urine culture. Ultrasonography revealed normal appearances of the liver and bile ducts, and normal-sized kidneys with a non-obstructing left-sided staghorn calculus. Leptospirosis was considered as a cause of his illness. Serological testing was requested and he was treated initially with piperacillin-tazobactam.
Within 12 hours of admission he became hypotensive with BP 60/40mmHg, developed new onset atrial fibrillation and his temperature rose to 38.5°C. He remained hypotensive despite receiving 4.5L of intravenous fluid, and was transferred to the critical care unit. He required circulatory support with metaraminol up to 70mg/hour and later norepinephrine up to 0.3mg/hour. He developed hemoptysis and his respiratory function quickly deteriorated necessitating non-invasive ventilation. Despite circulatory support, he became anuric and continuous renal replacement therapy was initiated. He was given intravenous hydrocortisone 200mg followed by intravenous methylprednisolone 500mg and doxycycline was added.
After two plasma exchange sessions, there was a dramatic reduction of the serum bilirubin to 10.5mg/dL (180μmol/L) and of the ALT to 2.7mg/dL (47μmol/L). His mental state and respiratory function also improved significantly. It was possible to gradually withdraw vasopressors and respiratory support and the patient became oriented and alert. Further plasma exchange sessions were not deemed necessary owing to this dramatic improvement. At this point, serological testing was negative for hepatitis A, B, C and E, human immunodeficiency virus, Epstein–Barr virus, cytomegalovirus and Borrelia burgdorferi. Initial serology for Leptospira IgM was also negative.
Seven days after starting plasma exchange, he was transferred to our renal unit for intermittent hemodialysis, and after 9 days his renal function had recovered sufficiently to stop renal replacement therapy. After 14 days, Leptospira IgM was tested as positive, followed by a positive Leptospira microscopic agglutination test (MAT) tested 30 days after presentation. The patient remains well, with normal liver function tests and a serum creatinine of 1.52mg/dL (135umol/L).
This patient developed severe sepsis with AKI and multi-organ failure 5 to 7 days after the onset of symptoms of Leptospira infection, coinciding with the immune phase of the disease. Notably, he had pulmonary involvement which is a predictor of poor outcome .
The most common mechanism of renal injury in leptospirosis is tubulointerstitial nephritis with interstitial edema and a cellular infiltrate. This typically manifests as non-oliguric AKI, with hypokalemia caused by resulting tubular dysfunction . In our case, there was oligo-anuria with no such electrolyte disturbance suggesting an alternative mechanism of AKI. As can be seen in Figure 1, initiation of plasma exchange led to an immediate improvement in serum bilirubin and ALT. Cardiovascular and respiratory function also improved dramatically allowing vasopressors and respiratory support to be withdrawn. His urine output improved 21 days after the onset of symptoms, followed by resolution of AKI.
Plasma exchange may disrupt the mechanisms causing tissue damage in severe sepsis.
Plasma exchange may prevent immune complex-mediated tissue injury.
Although vasculitis has been demonstrated in histological specimens from cases with severe leptospirosis, it has not been a persistent finding neither is it thought to be the primary pathological mechanism . In the immune phase of the disease, there is evidence for the development of pathogenic immune complexes and the disappearance of these complexes coincides with improvements in liver and kidney function . In murine models, activation of TLR by viral antigens can induce immune complex glomerulonephritis , but such a response has not been observed in leptospirosis. Instead, activation of TLR2 and TLR4 by LPS on renal epithelial cells is thought to cause tubulointerstitial nephritis [3, 8]. In addition, leptospiral OMP has also been shown to cause accumulation of extracellular matrix in tubular cells contributing to the development of renal fibrosis .
The pathophysiology of lung injury in severe leptospirosis is also unclear and is thought to be induced either by unidentified toxins or by an autoimmune mechanism. The deposition of IgM, IgA, IgG and C3 in the alveolus of patients with pulmonary hemorrhage secondary to leptospirosis and in a guinea pig model of severe pulmonary leptospirosis is similar to the pattern seen in Goodpasture’s syndrome [3, 9]. However, sera from patients with leptospirosis did not recognize the human glomerular basement membrane (GBM) and it is therefore unlikely that the lung disease seen in leptospirosis is caused by an anti-GBM antibody mechanism .
The serological diagnosis of leptospirosis remains challenging and in our case the diagnosis was made on the basis of the clinical picture. The available serological tests for Leptospira are an enzyme immunoassay (EIA), measuring leptospiral IgM, and a MAT. The EIA becomes positive from 5 days after infection, but can take longer, especially if antibiotics have been administered. Confirmation by MAT is recommended, which may be positive from the 10th day after the onset of symptoms .
In summary, our patient had features of severe leptospirosis, with AKI and pulmonary involvement. Despite predictors for poor outcome, his clinical condition and biochemical parameters improved dramatically after two plasma exchanges. Although it is possible that the patient recovered spontaneously with supportive management, his dramatic improvement leads us to believe that plasma exchange had a vital role in his recovery. We hypothesize that plasma exchange prevents injury mediated by sepsis or by an unknown immune mechanism in severe leptospirosis. In our experience, serological testing was not helpful due to the long delay to a positive result and we recommend treatment based on the clinical picture. We advocate the use of plasma exchange in deteriorating severe disease necessitating intensive care support despite standard treatment with antibiotics.
Written informed consent was obtained from the patient for publication of this case report and accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
Neither author has received any external funding for the preparation of this paper.
- Levett PN: Leptospirosis. Clin Microbiol Rev. 2001, 14 (2): 296-10.1128/CMR.14.2.296-326.2001.View ArticlePubMedPubMed CentralGoogle Scholar
- World Health Organisation: http://www.who.int/zoonoses/diseases/leptospirosis/en/ accessed December 2011
- Fraga TR, Barbosa AS, Isaac L: Leptospirosis: aspects of innate immunity, immunopathogenesis and immune evasion from the complement system. Scand J Immunol. 2010, 73: 408-419.View ArticleGoogle Scholar
- Watt G, Padre LP, Tuazon ML, Calubaquib C, Santiago E, Ranoa CP, Laughlin LW: Placebo-controlled trial of intravenous penicillin for severe and late leptospirosis. Lancet. 1988, 1 (8583): 433-View ArticlePubMedGoogle Scholar
- Kularatne SA, Budagoda BD, de Alwis VK, Wickramasinghe WM, Bandara JM, Pathirage LP, Gamlath GR, Wijethunga TJ, Jayalath WA, Jayasinghe C, Pinto V, Somaratne P, Kumarasiri PV: High efficacy of bolus methylprednisolone in severe leptospirosis: a descriptive study in Sri Lanka. Postgrad Med J. 2011, 87: 13-17. 10.1136/pgmj.2009.092734.View ArticlePubMedGoogle Scholar
- Trivedi SV, Vasava AH, Bhatia LC, Patel TC, Patel NK, Patel NT: Plasma exchange with immunosuppression in pulmonary alveolar haemorrhage due to leptospirosis. Indian J Med Res. 2010, 131: 429-433.PubMedGoogle Scholar
- Tse KC, Yip PS, Hui KM, Li FK, Yuen KY, Lai KN, Chan TM: Potential benefit of plasma exchange in treatment of severe icteric leptospirosis complicated by acute renal failure. Clin Vaccine Immunol. 2002, 9 (2): 482-484. 10.1128/CDLI.9.2.482-484.2002.View ArticleGoogle Scholar
- Cerdas-Quesada C: Potential benefits of plasma exchange by apheresis on the treatment of severe Icteric Leptospirosis: Case report and literature review. Transfus Apher Sci. 2011, 45 (2): 191-194. 10.1016/j.transci.2011.07.012.View ArticlePubMedGoogle Scholar
- Yang C-W, Wu M-S, Pan M-J: Leptospirosis renal disease. Nephrol Dial Transplant. 2001, 16 (Suppl. 5): 73-77.View ArticlePubMedGoogle Scholar
- Werts C: Leptospirosis: a Toll road from B lymphocytes. Chang Gung Med J. 2010, 33 (6): 591-601.PubMedGoogle Scholar
- van Deuren M, Frieling JTM, van der Ven-Jongekrijg J, Neeleman C, Russel FGM, van Lier HJJ, Bartelink AKM, van der Meer JWM: Plasma patterns of Tumour Necrosis Factor-α (TNF) and TNF soluble receptors during acute meningococcal infections and the effect of plasma exchange. Clin Infect Dis. 1998, 26: 918-923. 10.1086/513933.View ArticlePubMedGoogle Scholar
- Szczepiorkowski ZM, Bandarenko N, Kim HC, Linenberger ML, Marques MB, Sarode R, Schwartz J, Shaz BH, Weinstein R, Wirk A, Winters J: Guidelines on the use of therapeutic apheresis in clinical practice-evidence-based approach from the apheresis applications committee of the American Society for Apheresis. J Clin Apher. 2007, 22: 106-175. 10.1002/jca.20129.View ArticlePubMedGoogle Scholar
- Medeiros FR, Spichler A, Athanazie DA: Leptospirosis-associated disturbances of blood vessels, lungs and hemostasis. Acta Trop. 2010, 115: 155-162. 10.1016/j.actatropica.2010.02.016.View ArticleGoogle Scholar
- Galli M, Esposito R, Crocchiolo P, Chermotti M, Gasparro P, Dall’Agio P: Immune complexes in leptospirosis. Infection. 1985, 13: 156-10.1007/BF01642877.View ArticlePubMedGoogle Scholar
- Pawar RD, Patole PS, Zecher D, Segerer S, Kretzler M, Schlöndorff D, Anders HJ: Toll-like receptor-7 modulates immune complex glomerulonephritis. J Am Soc Nephrol. 2006, 17: 141-149. 10.1681/ASN.2005121335.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.