Palliative treatment of presacral recurrence of endometrial cancer using irreversible electroporation: a case report
© Niessen et al.; licensee BioMed Central Ltd. 2013
Received: 10 January 2013
Accepted: 27 March 2013
Published: 13 May 2013
Irreversible electroporation (IRE) is a new minimally invasive tumor ablation technique which induces irreversible disruption of cell membrane integrity by changing the transmembrane potential resulting in cell death. Irreversible electroporation is currently undergoing clinical investigation as local tumor therapy for malignant liver and lung lesions. This is the first case report to describe the successful palliative ablation of a presacral recurrence of an endometrial cancer to achieve locoregional tumor control and pain relief.
A 56-year-old Caucasian woman was referred for interventional treatment of an advanced local recurrence of endometrial cancer (11.9 × 11.6 × 14.9cm) with infiltration of the sacral bone and nerve plexus. Due to the immediate proximity to the sacral plexus, the patient could neither undergo surgical therapy nor a second radiation therapy. Due to its ablation mechanism irreversible electroporation was deemed to be the best therapy option.
We showed in this case that a large tumor mass adjacent to a bundle of neural structures, the sacral plexus, can be widely ablated by irreversible electroporation with only minor temporary impairment of the neural function, even though a large infiltrating tissue volume (941cm3) was ablated.
Irreversible electroporation (IRE) is a new non-thermal local ablative treatment procedure which induces the irreversible permeabilization of a membrane lipid bilayer by creation of nanopores resulting in cell death . IRE is currently undergoing clinical investigation as a locally ablative tumor therapy for different organ systems, such as kidney, lung or liver lesions .
Endometrial cancer represents the seventh most frequent tumor worldwide in women with an annual incidence of 142,000 patients worldwide . The standard therapy for endometrial cancer is total removal of the uterus, cervix, as much as the parametrial tissue as possible, and a wide margin of the vagina (Wertheim-Meigs operation). At advanced stage endometrial cancer adjuvant radiation is performed. Radiation is considered standard therapy of inoperable tumor stages .
This article reports the successful palliative ablation of a presacral recurrence of endometrial cancer as an individual palliative therapy trial in order to achieve locoregional tumor control and pain relief.
A 56-year-old Caucasian woman was referred for treatment of a local recurrence of endometrial cancer with infiltration of her sacral bone and nerve plexus. After initial diagnosis of endometrial cancer in 2005 (pT1B N0 MX G1 L0 V0), a Wertheim-Meigs operation was performed with subsequent radiotherapy with the initial result of complete tumor remission. In 2009 the patient presented with histologically confirmed pulmonary metastases. Under anti-hormonal therapy (medroxyprogesterone acetate), again complete remission was achieved. At the start of 2012, 7 years after the initial diagnosis, the patient was admitted to our hospital due to a presacral recurrence of the endometrial cancer with infiltration and widespread destruction of the os sacrum.
The patient’s case was discussed at the multidisciplinary tumor board for therapy options: due to the immediate proximity to the sacral plexus the patient could neither undergo surgical therapy nor a second radiation therapy because of the high risk of neural destruction potentially resulting in paralysis. Due to its ablation mechanism IRE was deemed to be the best therapy option for the patient as palliative disease control.
IRE uses a series of electrical pulses of microseconds to generate irreversible permeabilization of cell membranes and thereby induces cell death in the treated region. IRE seems to be highly effective in tissues with a high density of cell wall structures and less effective in tissues with a high concentration of collagenous and elastic fibers [5, 6]. This – in contrast to thermal ablation techniques like radiofrequency ablation (RFA) or microwave ablation – potentially allows tumor cell ablation without concomitant destruction of connective tissue, blood vessels and nerves . Due to this potentially selective cell ablation technique, IRE was offered as a palliative therapy option because it provided the opportunity of tumor mass reduction and decrease of tumor burden with reduced risk of impairment of the sacral plexus and surrounding blood vessels. The procedure with risks and benefits was discussed with the patient and informed consent was obtained.
Among the different tumor ablation techniques RFA is the most widespread technique . Even though percutaneous ablation techniques are used as possibly curative therapies, palliative tumor ablation can be useful to achieve locoregional control of tumor growth, pain relief or pain control, especially in patients with unresectable tumor manifestations .
Due to heat dissipation to adjacent structures there is an inherent risk of thermal damage of adjacent organs, blood vessels and, of course, nerves. Thus, lesions close to adjacent structures with high risk of unintended heat destruction still pose a challenge for percutaneous thermal ablation techniques. IRE, in contrast to RFA, is a non-thermal ablation technique of soft tissue and offers a possibility to overcome the aforementioned limitations of thermal ablations. Instead of using heat, IRE uses a series of electrical pulses for microseconds to generate irreversible permeabilization of cell membranes, presumably through the formation of nanoscale defects in the cell membrane, and thereby induces cell death in the treated tissue.
IRE originally was viewed as an undesirable side effect of reversible electroporation and therefore was studied only to define the upper limit of electrical parameters that induce reversible electroporation. Due to its various features, for example transdermal delivery or introduction of drugs and genes into cells, or electrochemotherapy, reversible electroporation is an important method in biotechnology and medicine . Davalos, Mir and Rubinsky in 2005 reported that IRE can be used as an independent modality for ablation of substantial tissue volumes . Their findings were subsequently confirmed in experimental studies on cells and in large animal models [13, 14].
In a series of studies, Lee could show that IRE produces irreversible tissue damage, which earlier was attributed only to thermal effects . Furthermore, IRE proved to be especially effective in tissues with a high density of cell wall structures and less effective in tissues with a high concentration of collagenous and elastic fibers, which is suggestive of a cell selective effect [5, 6]. There appears to be complete ablation up to the margin of blood vessels without compromising the functionality of the blood vessels . This – in contrast to thermal ablation – would allow tumor cell ablation without concomitant destruction of connective tissue, blood vessels and nerves, which means ablation of tumor cells in those areas where thermal ablation was not possible before. In the proximity of larger blood vessels thermal ablation techniques are also hindered by the heat-sink effect. Due to its cooling effect blood flow is an important determinant as much as a limiting factor of thermal ablation techniques [17, 18]. IRE seems to be unaffected by the blood flow and conversely does not potentially affect the macrovascularization of the ablation zone .
Due to its more selective and non-thermal ablation effect IRE widens the field of minimally invasive treatable lesions. We showed in this case report that a large malignant lesion adjacent to a bundle of neural structures, that is the sacral plexus, can be widely ablated by IRE with only minor, temporary impairment of the neural function, even though a large infiltrating tissue volume was ablated.
Written informed consent was obtained from the patient for publication of this case report and accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
- Freeman SA, Wang MA, Weaver JC: Theory of electroporation of planar bilayer membranes: predictions of the aqueous area, change in capacitance, and pore-pore separation. Biophys J. 1994, 67 (1): 42-56. 10.1016/S0006-3495(94)80453-9.View ArticlePubMedPubMed CentralGoogle Scholar
- Thomson KR, Cheung W, Ellis SJ, Federman D, Kavnoudias H, Loader-Oliver D, Roberts S, Evans P, Ball C, Haydon A: Investigation of the safety of irreversible electroporation in humans. J Vasc Interv Radiol. 2011, 22: 611-621. 10.1016/j.jvir.2010.12.014.View ArticlePubMedGoogle Scholar
- Parkin DM, Pisani P, Ferlay J: Global cancer statistics. CA Cancer J Clin. 1999, 49: 33-64. 10.3322/canjclin.49.1.33.View ArticlePubMedGoogle Scholar
- Amant F, Moerman P, Neven P, Timmermann D, Van Limbergen E, Vergote I: Treatment modalities in endometrial cancer. Curr Opin Oncol. 2007, 19: 479-485. 10.1097/CCO.0b013e32827853c0.View ArticlePubMedGoogle Scholar
- Lee EW, Loh CT, Kee ST: Imaging guided percutaneous irreversible electroporation: ultrasound and immunohistological correlation. Technol Cancer Res Treat. 2007, 6: 287-294.View ArticlePubMedGoogle Scholar
- Lee EW, Chen C, Prieto VE, Dry SM, Loh CT, Kee ST: Advanced hepatic ablation technique for creating complete cell death: irreversible electroporation. Radiology. 2010, 255 (2): 426-433. 10.1148/radiol.10090337.View ArticlePubMedGoogle Scholar
- Onik G, Rubinsky B, Mikus P: Irreversible electroporation: Implications for prostate ablation. Technol Cancer Res Treat. 2007, 6: 295-300.View ArticlePubMedGoogle Scholar
- Deodhar A, Dickfeld T, Single GW, Hamilton WC, Thornton RH, Sofocleous CT, Maybody M, Gonen M, Rubinsky B, Solomon SB: Irreversible electroporation near the heart: ventricular arrhythmias can be prevented with ECG synchronization. AJR Am J Roentgenol. 2011, 196: 330-335. 10.2214/AJR.10.4490.View ArticleGoogle Scholar
- Lencioni R, Crocetti L: Local-regional treatment of hepatocellular carcinoma. Radiology. 2012, 262: 43-58. 10.1148/radiol.11110144.View ArticlePubMedGoogle Scholar
- Mylona S, Karagiannis G, Patsoura S, Galani P, Pomoni M, Thanos L: Palliative treatment of rectal carcinoma recurrence using radiofrequency ablation. Cardiovasc Intervent Radiol. 2012, 35: 875-882. 10.1007/s00270-011-0320-x.View ArticlePubMedGoogle Scholar
- Heller R: Overview of electroporation. 2002. Technol Cancer Res Treat. 2002, 1: 317-318.View ArticlePubMedGoogle Scholar
- Davalos R, Mir L, Rubinsky B: Tissue ablation with irreversible electroporation. Annals Biomed Eng. 2005, 33: 223-231. 10.1007/s10439-005-8981-8.View ArticleGoogle Scholar
- Miller L, Leor J, Rubinsky B: Cancer cells ablation with irreversible electroporation. Technol Cancer Res Treat. 2005, 4: 699-706.View ArticlePubMedGoogle Scholar
- Ben-David E, Appelbaum L, Sosna J, Nissenbaum I, Goldberg SN: Characterization of irreversible electroporation ablation in in vivo porcine liver. Am J Roentgenol. 2012, 198: W62-W68. 10.2214/AJR.11.6940.View ArticleGoogle Scholar
- Lee RC: Cell injury by electric forces. Ann NY Acad Sci. 2005, 1066: 85-91. 10.1196/annals.1363.007.View ArticlePubMedGoogle Scholar
- Maor E, Ivorra A, Leor J, Rubinsky B: The effect of irreversible electroporation on blood vessels. Technol Cancer Res Treat. 2007, 6: 307-312.View ArticlePubMedGoogle Scholar
- Patterson EJ, Scudamore CH, Owen DA, Nagy AG, Buczkowski AK: Radiofrequency ablation of porcine liver in vivo: effects of blood flow and treatment time on lesion size. Ann Surg. 1998, 227: 559-565. 10.1097/00000658-199804000-00018.View ArticlePubMedPubMed CentralGoogle Scholar
- Charpentier KP: Irreversible electroporation for the ablation of liver tumors: are we there yet?. Arch Surg – Chicago. 2012, 147: 1053-1061. 10.1001/2013.jamasurg.100.View ArticlePubMedGoogle Scholar
- Kingham TP, Karkar AM, D’Angelica MI, Allen PJ, Dematteo RP, Getrajdman GI, Sofocleous CT, Solomon SB, Jarnagin WR, Fong Y: Ablation of perivascular hepatic malignant tumors with irreversible electroporation. J Am Coll Surg. 2012, 215 (3): 379-387. 10.1016/j.jamcollsurg.2012.04.029.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.