Figure 5

Histological and immunohistochemical evaluation of the deep area (group 3). (A) Hematoxylin and eosin staining showing hypercellular areas composed of round to ovoid epithelial cells, without nuclear pleomorphism or atypia (200× magnification). (B) Immunostaining with α-SMA was only detected in a few myoepithelial cells (100× magnification). (C) CK 8 was strongly expressed in most of the tumoral cells (200× magnification). (D) Positivity to p53 was detected in a few epithelial cells (200× magnification). (E) Immunostaining with p63 was only detected in a few myoepithelial cells (100× magnification). (F) CKs AE1/AE3 were strongly expressed in most of the tumoral cells (100× magnification). (G) Stronger expression of Ki67 antigen than in groups 1 and 2 was observed (100× magnification).